Figure 8 | Oncogene

Figure 8

From: Epidermal growth factor and serum activate distinct pathways to inhibit the BH3 only protein BAD in prostate carcinoma LNCaP cells

Figure 8

Inhibition of EGFR and ErbB2 activity do not prevent serum-mediated inhibition of LY-induced death in LNCaP cells. LNCaP cells serum-starved for 20 h were preincubated for 1 h without (control) or with 5 μ M AG1478 or 5 μ M AG879 before being treated with fresh medium containing 10% serum (serum), medium containing 10% serum and 25 μ M LY (LY+serum), serum-free medium with 25 μ M LY (LY) or serum-free medium containing 25 μ M LY and 100 ng/ml EGF (LY+EGF). (a) 8 h later caspase 3 activity was determined (b) 12 h later % of apoptotic cells was determined by propidium iodide staining as described in Materials and methods. Caspase 3 activity is expressed as time increase over cells incubated in serum alone (X increase versus control cells).

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