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  • Oncogenomics
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DNA copy number gains in head and neck squamous cell carcinoma

Oncogene volume 25, pages 1424–1433 (2006)Cite this article

Abstract

Gene amplification, a common mechanism for oncogene activation in cancer, has been used as a tag for the identification of novel oncogenes. DNA amplification is frequently observed in head and neck squamous cell carcinoma (HNSCC) and potential oncogenes have already been reported. We applied restriction landmark genome scanning (RLGS) to study gene amplifications and low-level copy number changes in HNSCC in order to locate previously uncharacterized regions with copy number gains in primary tumor samples. A total of 63 enhanced RLGS fragments, indicative of DNA copy number changes, including gains of single alleles, were scored. Enhanced sequences were identified from 33 different chromosomal regions including those previously reported (e.g. 3q26.3 and 11q13.3) as well as novel regions (e.g. 3q29, 8q13.1, 8q22.3, 9q32, 10q24.32, 14q32.32, 17q25.1 and 20q13.33). Furthermore, our data suggest that amplicons 11q13.3 and 3q26.3–q29 may be divided into possibly two and three independent amplicons, respectively, an observation supported by published microarray expression data.

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Acknowledgements

We thank Kristi Bennett for her critical revisions and discussions of the manuscript. This work was supported in part by a grant from NIDCR, DE13123-02 (CP).

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Authors and Affiliations

  1. Department of Molecular Virology, Division of Human Cancer Genetics, Immunology and Medical Genetics, The Ohio State University, Columbus, OH, USA

    L T Smith & C Plass

  2. Deparment of Otolaryngology and Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA

    M Lin, J C Lang & D E Schuller

  3. Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University, Columbus, OH, USA

    M Lin

  4. Department of Cancer Genetics and Comprehensive Cancer Center, Roswell Park Cancer Institute, Buffalo, NY, USA

    D J Smiraglia

  5. Computer Information Sciences Department, The Ohio State University, Columbus, OH, USA

    R Kazhiyur-Mannar & R Wenger

  6. Emeritus Professor of Sensory Biophysics, The Ohio State University, Columbus, OH, USA

    K Kornacker

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  1. M Lin
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Correspondence to C Plass.

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Lin, M., Smith, L., Smiraglia, D. et al. DNA copy number gains in head and neck squamous cell carcinoma. Oncogene 25, 1424–1433 (2006). https://doi.org/10.1038/sj.onc.1209166

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