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A 1 Mb minimal amplicon at 8p11–12 in breast cancer identifies new candidate oncogenes

Oncogene volume 24, pages 5235–5245 (2005)Cite this article

Abstract

Amplification of 8p11–12 is a well-known alteration in human breast cancers but the driving oncogene has not been identified. We have developed a high-resolution comparative genomic hybridization array covering 8p11–12 and analysed 33 primary breast tumors, 20 primary ovarian tumors and 27 breast cancer cell lines. Expression analysis of the genes in the region was carried out by using real-time quantitative PCR and/or oligo-microarray profiling. In all, 24% (8/33) of the breast tumors, 5% (1/20) of the ovary tumors and 15% (4/27) of the cell lines showed 8p11–12 amplification. We identified a 1 Mb segment of common amplification that excludes previously proposed candidate genes. Some of the amplified genes did not show overexpression, whereas for others, overexpression was not specifically attributable to amplification. The genes FLJ14299, C8orf2, BRF2 and RAB11FIP, map within the 8p11–12 minimal amplicon, two have a putative function consistent with an oncogenic role, these four genes showed a strong correlation between amplification and overexpression and are therefore the best candidate driver oncogenes at 8p12.

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Acknowledgements

We thank Celine Courtay-Cahen for characterization of the BACs, Rachel Lyman for preparation of cell lines, Ahmed Ahmed for revision of ovarian tumors staging and grading, Ian Giddings and Jeremy Clark for help printing the CGH-array and David Bowtell for reviewing the manuscript. This work was supported by Cancer Research UK (CRUK), Wellbeing, the Biotechnology and Biological Sciences Research Council (BBSRC) and The Cambridge-MIT Institute (CMI). JDB is a CRUK Senior Clinical Research Fellow.

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Authors and Affiliations

  1. Department of Oncology, Hutchison/MRC Research Centre, Cancer Genomics Program, University of Cambridge, Hills Road, Cambridge, CB2 2XZ, UK

    Maria J Garcia, Suet-Feung Chin, Andrew Teschendorff, Ali Naderi, Hilal Ozdag, Maria Vias, Tanja Kranjac, Tatiana Subkhankulova, James D Brenton & Carlos Caldas

  2. Department of Pathology, Hutchison/MRC Research Centre, University of Cambridge, Hills Road, Cambridge, CB2 2XZ, UK

    Jessica C M Pole & Paul A W Edwards

  3. Department of Histopathology, The Breast Unit, Nottingham City Hospital NHS Trust and University of Nottingham, Nottingham, NG5 1PB, UK

    Claire Paish & Ian Ellis

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  1. Maria J Garcia
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Correspondence to Carlos Caldas.

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Supplementary Information accompanies the paper on Oncogene website (http://www.nature.com/onc)

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Garcia, M., Pole, J., Chin, SF. et al. A 1 Mb minimal amplicon at 8p11–12 in breast cancer identifies new candidate oncogenes. Oncogene 24, 5235–5245 (2005). https://doi.org/10.1038/sj.onc.1208741

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