Figure 5 | Oncogene

Figure 5

From: The glucose dependence of Akt-transformed cells can be reversed by pharmacologic activation of fatty acid β-oxidation

Figure 5

Akt activation affects AMPK-dependent changes in lipid metabolism following glucose withdrawal. (a) Fatty acid oxidation was determined by measuring 3H2O produced by control LN229 cells (control) or LN229 cells expressing myrAkt (myrAkt) incubated with [9,10-3H]palmitate for 6 h. Cells were incubated in serum-free medium containing glucose (+glc), no glucose (−glc), or no glucose and 1 mM AICAR (−glc+AICAR). Data are presented as mean±s.d. of triplicate samples and are representative of three independent experiments. (b) Fatty acid synthesis was assayed by measuring the amount of 14C incorporated into lipids after 2 h incubation with [1-14C]acetate. Prior to incubation with [1-14C]acetate, vector control and Akt-expressing cells were plated in medium containing glucose (+glc), no glucose (−glc), or no glucose and 1 mM AICAR (−glc+AICAR) for 1 h. (c) Fatty acid oxidation was measured in LN229+myrAkt parental cells (myrAkt+control) and cells expressing a dominant-negative form of AMPK (myrAkt+dnAMPK). Cells were cultured in the absence of glucose in the presence (−glc+AICAR) or absence (−glc) of 1 mM AICAR. Data are presented as mean±s.d. of triplicate samples and are representative of three independent experiments