Figure 4 | Oncogene

Figure 4

From: The glucose dependence of Akt-transformed cells can be reversed by pharmacologic activation of fatty acid β-oxidation

Figure 4

Rapamycin treatment blocks phosphorylation of 4EBP1 but does not protect Akt-expressing cells from glucose deprivation-induced death. (a) Control LN229 cells or LN229 cells expressing myrAkt were maintained in serum-free medium for 24 h before they were withdrawn from glucose in the presence or absence of 1 mM AICAR or 20 nM rapamycin (RAP) for 2 h. Lysates were analysed by Western blot for phospho-Ser473 Akt (pS473-Akt), phospho-Thr37/46 4EBP1 (pT37/46-4EBP1), and actin. Percent viability was measured daily for control LN229 cells (b), or LN229 cells expressing myrAkt (c), when cultured in medium containing no glucose in the presence of no drug (−glc; ), 20 nM rapamycin (−glc+RAP; •), or 1 mM AICAR (−glc+AICAR; □). Data are presented as mean±s.d. of triplicate samples and are representative of three independent experiments

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