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In vitro and in vivo analysis of the interaction between 5.8S rRNA and ARF protein reveal a new difference between murine p19ARF and human p14ARF

Oncogene volume 24, pages 2580–2584 (2005)Cite this article

Abstract

Both human and murine ARF proteins have been recently reported to impair rRNA maturation and ribosomes biogenesis through a p53-independent pathway. A specific interaction has been established between 5.8S rRNA and the murine p19ARF specie. We report here, by use of both in vitro and ChIP-RNA assays, the absence of any interaction between the human p14ARF and the homologous 5.8S rRNA. Our data are not consistent with the involvement of a 5.8S–p14ARF complex in ribosome biogenesis in man. Rather they suggest that the human protein does not require such an interaction to achieve a similar function. This result is a new argument in favour of functional differences between human and murine ARF proteins.

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Acknowledgements

This work was supported by a grant from the ‘Ligue contre le Cancer, Comité de la Vienne’. L Andrique and O Ayrault are supported by grants from the ‘Ligue Nationale contre le Cancer’ and the ‘Fondation pour la Recherche Médicale’, respectively. We are grateful to CJ Sherr for providing us with the HA-p19ARF cDNA.

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  1. EA 3805, Laboratoire d'Oncologie Moléculaire, Pôle Biologie-Santé, 40 avenue du Recteur Pineau, 86022, Poitiers Cedex, France

    Laëtitia Andrique, Olivier Ayrault, Christian-Jacques Larsen & Paule Séité

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  1. Laëtitia Andrique
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  2. Olivier Ayrault
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Correspondence to Paule Séité.

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Andrique, L., Ayrault, O., Larsen, CJ. et al. In vitro and in vivo analysis of the interaction between 5.8S rRNA and ARF protein reveal a new difference between murine p19ARF and human p14ARF. Oncogene 24, 2580–2584 (2005). https://doi.org/10.1038/sj.onc.1208457

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