Figure 7 | Oncogene

Figure 7

From: Expression of a constitutively active mutant of M-Ras in normal bone marrow is sufficient for induction of a malignant mastocytosis/mast cell leukemia, distinct from the histiocytosis/monocytic leukemia induced by expression of activated H-Ras

Figure 7

Transplantation of bone-marrow cells expressing activated M-Ras or H-Ras resulted respectively, in a malignant mastocytosis or histiocytosis. Bone-marrow cells transduced with retroviruses expressing GFP alone or constitutively active M-Ras or H-Ras plus GFP were selected for by culture for 48 h in the presence of puromycin. 1 × 106 cells were injected intravenously into groups of sublethally irradiated (800 rads) Balb/c mice. Mice were killed and dissected when they became moribund, at 8- or 2 weeks respectively for cells expressing activated M-Ras or H-Ras. (a) The livers and spleens were fixed in 4% paraformaldehyde and the paraffin-embedded samples were sectioned and stained with either Toluidine Blue (Q71L M-Ras), showing infiltration by granulated mast cells, or hematoxylin and eosin (G12V H-Ras), showing infiltration by nongranulated mononuclear cells. (b) Flow cytometric analyses using antibodies against the indicated antigens of cell suspensions of spleens from mice injected with the bone-marrow cells expressing activated M-Ras or H-Ras as indicated

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