Abstract
Cyclin A1 is an alternative A-type cyclin that is essential for spermatogenesis, but it is also expressed in hematopoietic progenitor cells and in acute myeloid leukemia. Its functions during cell cycle progression of somatic cells are incompletely understood. Here, we have analysed the cell cycle functions of cyclin A1 in transformed and nontransformed cells. Murine embryonic fibroblasts derived from cyclin A1-deficient mice were significantly impaired in their proliferative capacity. In accordance, cyclin A1−/− cells accumulated in G1 and G2/M phase while the percentage of S phase cells decreased. Also, lectin stimulated splenic lymphocytes from cyclin A1−/− mice proliferated slower than their wild-type counterparts. Forced cyclin A1 overexpression in NIH3T3 cells and in U937 leukemic cells either by transient transfection or by retroviral infection enhanced S phase entry. Consequently, siRNA mediated silencing of cyclin A1 in highly cyclin A1 expressing ML1 leukemic cells significantly slowed S phase entry, decreased proliferation and inhibited colony formation. Taken together, these analyses demonstrate that cyclin A1 contributes to G1 to S cell cycle progression in somatic cells. Cyclin A1 overexpression enhances S phase entry consistent with an oncogenic function. Finally, cyclin A1 might be a therapeutic target since its silencing inhibited leukemia cell growth.
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References
Berthet C, Aleem E, Coppola V, Tessarollo L and Kaldis P . (2003). Curr. Biol., 13, 1775–1785.
Coletta RD, Christensen K, Reichenberger KJ, Lamb J, Micomonaco D, Huang L, Wolf DM, Müller-Tidow C, Golub TR, Kawakami K and Ford HL . (2004). Proc. Natl. Acad. Sci. USA, 101, 6478–6483.
Diederichs S, Bäumer N, Ji P, Metzelder SK, Idos GE, Cauvet T, Wang W, Gromoll J, Schrader MG, Koeffler HP, Berdel WE, Serve H and Müller-Tidow C . (2004). J. Biol. Chem., 279, 33727–33741.
Dyson N . (1998). Genes Dev., 12, 2245–2262.
Geng Y, Yu Q, Sicinska E, Das M, Schneider JE, Bhattacharya S, Rideout WM, Bronson RT, Gardner H and Sicinski P . (2003). Cell, 114, 431–443.
Helin K . (1998). Curr. Opin. Genet. Dev., 8, 28–35.
Knudson AG . (2002). Am. J. Med. Genet., 111, 96–102.
Lania L, Majello B and Napolitano G . (1999). J. Cell. Physiol., 179, 134–141.
Liao C, Wang XY, Wei HQ, Li SQ, Merghoub T, Pandolfi PP and Wolgemuth DJ . (2001). Proc. Natl. Acad. Sci. USA, 98, 6853–6858.
Liu D, Matzuk MM, Sung WK, Guo Q, Wang P and Wolgemuth DJ . (1998). Nat. Genet., 20, 377–380.
Müller C, Yang R, Park DJ, Serve H, Berdel WE and Koeffler HP . (2000). Blood, 96, 3894–3899.
Müller-Tidow C, Steffen B, Cauvet T, Tickenbrock L, Ji P, Diederichs S, Sargin B, Köhler G, Stelljes M, Puccetti E, Ruthardt M, deVos S, Hiebert SW, Koeffler HP, Berdel WE and Serve H . (2004). Mol. Cell. Biol., 24, 2890–2904.
Müller-Tidow C, Wang W, Idos GE, Diederichs S, Yang R, Readhead C, Berdel WE, Serve H, Saville M, Watson R and Koeffler HP . (2001). Blood, 97, 2091–2097.
Ortega S, Prieto I, Odajima J, Martin A, Dubus P, Sotillo R, Barbero JL, Malumbres M and Barbacid M . (2003). Nat. Genet., 35, 25–31.
Parisi T, Beck AR, Rougier N, McNeil T, Lucian L, Werb Z and Amati B . (2003). EMBO J., 22, 4794–4803.
Resnitzky D, Gossen M, Bujard H and Reed SI . (1994). Mol. Cell. Biol., 14, 1669–1679.
Sweeney C, Murphy M, Kubelka M, Ravnik SE, Hawkins CF, Wolgemuth DJ and Carrington M . (1996). Development, 122, 53–64.
Van der Meer T, Chan WY, Palazon LS, Nieduszynski C, Murphy M, Sobczak-Thepot J, Carrington M and Colledge WH . (2004). Reproduction, 127, 503–511.
Weinberg RA . (1995). Cell, 81, 323–330.
Yang R, Morosetti R and Koeffler HP . (1997). Cancer Res., 57, 913–920.
Yang R, Müller C, Huynh V, Fung YK, Yee AS and Koeffler HP . (1999a). Mol. Cell. Biol., 19, 2400–2407.
Yang R, Nakamaki T, Lübbert M, Said J, Sakashita A, Freyaldenhoven BS, Spira S, Huynh V, Müller C and Koeffler HP . (1999b). Blood, 93, 2067–2074.
Acknowledgements
This work is supported by grants from the Deutsche Forschungsgemeinschaft (Mu 1328/2-3, Se 600/3), the Deutsche Krebshilfe (10-1539-Mü3), the IMF and the IZKF at the University of Münster. C. Müller-Tidow is supported by the DFG-Heisenberg program (Mu1328/3-1).
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Ji, P., Agrawal, S., Diederichs, S. et al. Cyclin A1, the alternative A-type cyclin, contributes to G1/S cell cycle progression in somatic cells. Oncogene 24, 2739–2744 (2005). https://doi.org/10.1038/sj.onc.1208356
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DOI: https://doi.org/10.1038/sj.onc.1208356
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