Figure 1 | Oncogene

Figure 1

From: Redistribution of CD95, DR4 and DR5 in rafts accounts for the synergistic toxicity of resveratrol and death receptor ligands in colon carcinoma cells

Figure 1

Differential sensitivity of colon carcinoma cell lines to resveratrol-induced apoptosis. Indicated human colon cancer cell lines were left untreated or treated for 72 h with 30 (R30), 50 (R50) or 100 (R100) μ M resveratrol before measuring the percentage of apoptosis in cells stained with Hoechst 33342 (mean ±s.d. of three independent experiments; 300 cells/point). Inset – Upper panel: Flow cytometry analysis of active caspase-3 in HT29 and SW480 cells left untreated (Co) or treated for 72 h with 30 μ M resveratrol (R30). Gray areas: Isotype-matched control Ab. White areas: Antiactive caspase-3 Ab. Lower panel: Western blot analysis of the 116 kDa PARP and its 89 kDa cleavage fragment. The 42 kDa actin was used for loading control

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