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  • Oncogenomics
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Prediction of high risk Ewing's sarcoma by gene expression profiling

Oncogene volume 23, pages 8997–9006 (2004)Cite this article

A Corrigendum to this article was published on 16 June 2005

Abstract

Ewing's sarcoma (ES) is the second most common primary malignant bone tumor in children and adolescents. Currently accepted clinical prognostic factors fail to classify ES patients' risk to relapse at diagnosis. We aimed to find a new strategy to distinguish between poor and good prognosis ES patients already at diagnosis. We analysed the gene expression profiles of 14 primary tumor specimens and six metastases from ES patients, using oligonucleotide microarray analysis. The over-expression of two genes was validated by quantitative PCR using the LightCycler system. We identified two distinct gene expression signatures distinguishing high-risk ES patients that are likely to progress from low-risk ES patients with a favorable prognosis of long-term progression-free survival. The microarray-based classification was superior to currently used prognostic parameters. Over-expressed genes in the poor prognosis patients included genes regulating the cell cycle and genes associated with invasion and metastasis, while among the downregulated genes were tumor suppressor genes and inducers of apoptosis. Our results indicate the existence of a specific gene expression signature of outcome in ES already at diagnosis, and provide a strategy to select patients who would benefit from risk-adapted improved therapy.

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Acknowledgements

This work was supported by the Josefina Maus and Gabriela Cesarman Maus Chair for Pediatric Hematology Oncology (RZ). This work was performed in partial fulfillment of the requirements for the PhD degree of Anat Ohali, Sackler School of Medicine, Tel-Aviv University, Israel.

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Authors and Affiliations

  1. Molecular Oncology, Felsenstein Medical Research Center, Rabin Medical Campus, 39 Jabotinski Street, Petah Tikva 49100, Israel and Sackler Faculty of Medicine, Tel Aviv University, POB 39040, Tel Aviv, 69978, Israel

    Anat Ohali, Smadar Avigad, Rina Zaizov & Isaac Yaniv

  2. Pediatric Hematology Oncology, Schneider Children's Medical Center of Israel, 14 Kaplan Street, Petah Tikva 49202, Israel and Sackler Faculty of Medicine, Tel Aviv University, POB 39040, Tel Aviv, 69978, Israel

    Smadar Avigad, Rina Zaizov, Ian J Cohen & Isaac Yaniv

  3. Department of Biological Services, DNA Array Unit, Bioinformatics, Weizmann Institute of Science, POB 26, Rehovot, 76100, Israel

    Ron Ophir & Shirley Horn-Saban

  4. Sourasky Medical Center, 6 Weizmann Street, Tel Aviv 64239, Israel and Sackler Faculty of Medicine, Tel Aviv University, POB 39040, Tel Aviv, 69978, Israel

    Isaac Meller, Yehuda Kollender & Josephine Issakov

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  1. Anat Ohali
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Correspondence to Smadar Avigad.

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Ohali, A., Avigad, S., Zaizov, R. et al. Prediction of high risk Ewing's sarcoma by gene expression profiling. Oncogene 23, 8997–9006 (2004). https://doi.org/10.1038/sj.onc.1208060

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