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  • Oncogenomics
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Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer

Oncogene volumeĀ 23,Ā pages 7734ā€“7745 (2004)Cite this article

Abstract

Expression array data for >3000 individual clones from two suppression subtractive hybridization libraries revealed 147 genes overexpressed in non-small-cell lung cancer (NSCLC) cell lines. Of these 147 genes, 30 genes have previously unknown cancer association and 65 genes have been associated with cancers other than NSCLC. The identification of 52 genes previously associated with NSCLC by different methodologies supports the validity of the strategy used here. Of the 147 genes, 19 have no prior named Unigene cluster designation, and are designated herein as L1 to L19. Quantitative real-time PCR and cancer profiling arrays were used as independent validation tools to confirm tumor overexpression for five of the ā€˜Lā€™ genes in tumor cell lines and patient samples from NSCLC and other cancers. Follow-up studies for candidate NSCLC-associated genes can be useful in providing valuable insight into the etiology of lung cancer as well as providing potentially interesting diagnostic or therapeutic targets for further investigation.

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Abbreviations

AC:

adenocarcinoma

ASCC:

adenosquamous cell carcinoma

BAC:

bronchioalveolar carcinoma

CPA:

cancer profiling array

GFP:

green fluorescent protein

LCC:

large-cell carcinoma

NSCLC:

non-small-cell lung cancer

QPCR:

quantitative real-time PCR

SAGE:

serial analysis of gene expression

SCC:

squamous cell carcinoma

SCLC:

small-cell lung cancer

SSH:

suppression subtractive hybridization

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Acknowledgements

We thank our colleagues, Michelle Hering and Stephanie James, for assistance in the culturing of NSCLC cell lines for RNA preparation.

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  1. Department of Antibody Technologies, Seattle Genetics Inc., 21823 30th Drive SE, Bothell, 98021, WA, USA

    Joseph Petroziello,Ā Andrew Yamane,Ā Lori Westendorf,Ā Melissa Thompson,Ā Charlotte McDonaghĀ &Ā Paul Carter

  2. Department of Biochemistry, Seattle Genetics Inc., 21823 30th Drive SE, Bothell, 98021, WA, USA

    Charles Cerveny,Ā Che-Leung LawĀ &Ā Alan Wahl

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Petroziello, J., Yamane, A., Westendorf, L. et al. Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer. Oncogene 23, 7734ā€“7745 (2004). https://doi.org/10.1038/sj.onc.1207921

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