Figure 3 | Oncogene

Figure 3

From: DKK1, a negative regulator of Wnt signaling, is a target of the β-catenin/TCF pathway

Figure 3

Binding capacity of DKK1 TBEs to TCF protein. Duplex oligonucleotides (6 ng) encompassing each of four TBEs were incubated with GST-fusion protein (0.5 μg) containing the HMG domain of human TCF4E. DNA-protein interactions were analysed by gel shift assays. Excess amounts of unlabeled probes (300 ng) containing either wild-type or mutated TBEs (5′-CTTTG[A/T][A/T]-3′ → 5′-CTTTGGC-3′) were used as competitors in several reactions

Back to article page