Figure 7 | Oncogene

Figure 7

From: p53 functions in the incorporation step in DNA base excision repair in mouse liver mitochondria

Figure 7

Pol γ activity stimulation by p53: increasing amounts of mitochondrial extracts (0.25–5 μg) from wild-type (wt) or p53 knockout (p53−/−) mice were incubated with 1 pmol of a 1 nucleotide gap oligonucleotide, in the presence of 32P-dCTP, and gap-filing activity was quantified as the amount of radioactivity incorporated, relative to 0.25 μg of MLME-wt (a). Alternatively, 5 μg of MLME from wt or p53−/− mice were incubated with 1 pmol of gap substrate alone or with increasing concentrations of recombinant p53 (b). Gap-filing activity was quantified as above, relative to incorporation with 5 μg of MLME-wt in the absence of added p53. Results presented are average±SEM of three and two separate experiments

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