Figure 3 | Oncogene

Figure 3

From: p53 functions in the incorporation step in DNA base excision repair in mouse liver mitochondria

Figure 3

p53 stimulation of BER in mitochondrial and nuclear mouse liver extracts: 50 μg of nuclear (a) or mitochondrial (b) extracts from wild type (lanes 1–4) or p53 knockout (lanes 5–8) were incubated with C30 (lanes 1 and 5) or U30 (lanes 2–4 and 6–8) oligonucleotides for 2 h at 32°C, in the absence or presence of 500 ng or 1 μg of recombinant p53. (c) 50 μg of wt (lanes 1 and 2) or p53−/− (lanes 3–6) mitochondrial extracts were incubated with C30 (lane 1) or U30 (lanes 3–6) oligonucleotides for 2 h at 32°C, with the following additions: 0.5 μg of recombinant p53 (lane 4), 0.5 μg of heat-denatured p53 (lane 5) or 1 μg of BSA (lane 6). The graphs present the mean±standard deviation of two animals per genotype, each assayed twice

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