Figure 2 | Oncogene

Figure 2

From: p53 functions in the incorporation step in DNA base excision repair in mouse liver mitochondria

Figure 2

Uracil-initiated repair synthesis incorporation in mitochondrial and nuclear mouse liver extracts: Repair incorporation reactions were carried out with undamaged (C30, lanes 2–4) or uracil-containing (U30, lanes 5–7) 30-mer double-strand oligonucleotides, with 50 μg of mitochondrial (a) or nuclear (b) extracts for 4 or 2 h, respectively, at 32°C. The DNA was precipitated and resolved in a 20% PAGE and the band intensities were quantified. Relative incorporation was calculated relative to the incorporation obtained with wild-type extracts incubated with uracil-containing oligo. Radioactivity present in control lanes was subtracted from values obtained with damaged substrate. Results presented are the mean±standard deviation of three animals per genotype

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