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  • Oncogenomics
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Molecular cloning, characterization and isolation of novel spliced variants of the human ortholog of a rat estrogen-regulated membrane-associated protein, UO-44

Oncogene volume 23, pages 5707–5718 (2004)Cite this article

A Corrigendum to this article was published on 18 November 2004

Abstract

We have previously reported the characterization of an estrogen-regulated rat uterine–ovarian-specific complementary DNA (UO-44). To understand the involvement of this protein in the initiation and progression of human ovarian and uterine cancers, we now report the cloning and characterization of the human ortholog (HuUO-44). HuUO-44 is mapped to chromosome 10q26.13 and contains nine exons. Multiple tissue Northern blot detected two HuUO-44 transcripts of approximately 2 and 3 kb in the pancreas. RT–PCR demonstrated that HuUO-44 undergoes a complex series of alternative splicing events between exons 2 and 6 that yielded four novel splice variants, HuUO-44A, HuUO-44B, HuUO-44C and HuUO-44D. Putative functional motifs identified in HuUO-44 are two CUB domains and a zona-pellucida domain. Transfection studies demonstrated the membrane-associated nature of HuUO-44. By immunohistochemistry, HuUO-44 was located to the normal ovarian and ovarian tumor epithelial cells; in NIH-OVCAR3 ovarian cancer cells, HuUO-44 was detected only at the leading edge of the dividing cells. Most importantly, a marked loss in cell attachment and proliferation was observed in NIH-OVCAR3 cells cultured in the presence of a polyclonal HuUO-44 antiserum. These findings suggest the potential role of HuUO-44 in cell motility, cell–cell interactions and/or interactions with the extracellular matrices.

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Acknowledgements

This work was supported by grants from National Medical Research Council of Singapore (NMRC/0541/2001), A*STAR-BMRC (LS/00/017) and A*STAR-BMRC (LS/00/019) to Huynh Hung.

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Authors and Affiliations

  1. Division of Cellular and Molecular Research, Laboratory of Molecular Endocrinology, National Cancer Centre of Singapore, Singapore, 169610, Singapore

    Caine Tuck Choy Leong, Chuan Young Ng, Chee Pang Ng, Zeng Shuan Ma, Thanh Hung Nguyen & Hung Huynh

  2. Department of Obstetrics & Gynaecology, Singapore General Hospital, Singapore, 169608, Singapore

    Sun Kuie Tay

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  1. Caine Tuck Choy Leong
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  2. Chuan Young Ng
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Correspondence to Hung Huynh.

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Leong, C., Ng, C., Ng, C. et al. Molecular cloning, characterization and isolation of novel spliced variants of the human ortholog of a rat estrogen-regulated membrane-associated protein, UO-44. Oncogene 23, 5707–5718 (2004). https://doi.org/10.1038/sj.onc.1207754

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