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Sil overexpression in lung cancer characterizes tumors with increased mitotic activity

Oncogene volume 23, pages 5371–5377 (2004)Cite this article

Abstract

Sil (SCL interrupting locus) was cloned from the most common chromosomal rearrangement in T-cell acute lymphoblastic leukemia. It is an immediate early gene whose expression is associated with cell proliferation. Sil protein levels are tightly regulated during the cell cycle, reaching peak levels in mitosis and disappearing on transition to G1. A recent study found Sil to be one of 17 genes whose overexpression in primary adenocarcinomas predicts metastatic spread. We hypothesized that Sil might have a role in carcinogenesis. To address this question, we utilized several approaches. Using a multitumor tissue array, we found that Sil protein expression was increased mostly in lung cancer, but also at lower levels, in a subset of other tumors. Microarray gene expression analysis and immunohistochemistry of lung cancer samples verified these observations. Sil gene expression in lung cancer correlated with the expression of several kinetochore check-point genes and with the histopathologic mitotic index. These observations suggest that overexpression of the Sil gene characterizes tumors with increased mitotic activity.

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Acknowledgements

We thank Sergey Nemez for the statistical analysis. This study was supported by research grants from the Israel Science Foundation, the Israel Cancer Research foundation and from Tel-Aviv University Recanati foundation to S Izraeli. N Kaminski is the Dorothy P and Richard P Simmons chair of Interstitial Lung Diseases at the University of Pittsburgh Medical School. G Rechavi holds the Gregorio and Dora Shapiro Chair for Hematology Malignancies, Sackler School of Medicine, Tel-Aviv University. This work was preformed in partial fulfillment of the requirements for the PhD degree of Ayelet Erez, Sackler Faculty of Medicine, Tel-Aviv University.

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Authors and Affiliations

  1. Department of Pediatric Hemato-Oncology, The Chaim Sheba Cancer Research Center, Sheba Medical Center, Tel-Hashomer, Israel

    Ayelet Erez, Inna Muler, Gideon Rechavi & Shai Izraeli

  2. Department of Pathology, The Chaim Sheba Cancer Research Center, Sheba Medical Center, Tel-Hashomer, Israel

    Marina Perelman & Iris Goldberg

  3. Advanced Technologies Center, NIH, Bethesda, MD, USA

    Stephen M Hewitt

  4. Department of Functional Genomics, The Chaim Sheba Cancer Research Center, Sheba Medical Center, Tel-Hashomer,

    Gadi Cojacaru, Iris Shahar, Ninette Amariglio, Gideon Rechavi & Naftali Kaminski

  5. Compugen LTD, Tel-Aviv, Israel

    Gadi Cojacaru

  6. Department of Pulmonary Medicine, The Chaim Sheba Cancer Research Center, Sheba Medical Center, Tel-Hashomer, Israel

    Marina Perelman, Gadi Cojacaru, Pnina Yaron, Meir Krupsky & Naftali Kaminski

  7. Sackler Faculty of Medicine, Tel-Aviv University, Israel

    Ayelet Erez, Pnina Yaron, Gideon Rechavi, Meir Krupsky, Naftali Kaminski & Shai Izraeli

  8. Geneic Branch, National Cancer Institute, NIH, Bethesda, MD, USA

    Stefano Campaner & Ilan R Kirsch

  9. The Dorothy P and Richard P Simmons Center, University of Pittsburgh Medical Center, Pittsburgh, PA, USA

    Naftali Kaminski

Authors
  1. Ayelet Erez
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  2. Marina Perelman
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  3. Stephen M Hewitt
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  4. Gadi Cojacaru
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  7. Pnina Yaron
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  8. Inna Muler
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  9. Stefano Campaner
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  10. Ninette Amariglio
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  12. Ilan R Kirsch
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  13. Meir Krupsky
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  14. Naftali Kaminski
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  15. Shai Izraeli
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Corresponding authors

Correspondence to Naftali Kaminski or Shai Izraeli.

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Erez, A., Perelman, M., Hewitt, S. et al. Sil overexpression in lung cancer characterizes tumors with increased mitotic activity. Oncogene 23, 5371–5377 (2004). https://doi.org/10.1038/sj.onc.1207685

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  • DOI: https://doi.org/10.1038/sj.onc.1207685

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