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Aurora B -TACC1 protein complex in cytokinesis

Oncogene volume 23, pages 4516–4522 (2004)Cite this article

Abstract

Taxins are a family of centrosomal proteins important for the regulation of mitosis and microtubule dynamics. Cytokinesis, the last step of M phase, is essential for chromosomal integrity and cell division. It is highly regulated and involves a reorganization of microtubules and actin filaments. We show here that TACC1 localizes diffusely to the midzone spindle in anaphase and strongly to the midbody during cytokinesis, indicating a possible involvement of this protein in the exit of M phase. TACC1 also relocalizes to the nucleolus in interphase. We demonstrate that TACC1 and the mitotic kinase Aurora B belong to the same complex during cytokinesis. We further show that Aurora B knocked down by RNA-mediated interference prevents the formation of the midbody – and consequently affects TACC1 localization at this site – and leads to abnormal cell division and multinucleated cells.

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Acknowledgements

We thank J-P Borg, F Coulier, C Ginestier, J Jacquemier and R Roubin for discussions, JP Borg for the gift of anti-scribble antibody, and A Gonçalvès, JR Galindo and D Isnardon for occasional help. This work was supported by INSERM, Institut Paoli-Calmettes and la Ligue Nationale contre le Cancer (Label). BD and AF are recipients of a fellowship from the Ministry of Research and NC from Association pour la Recherche contre le Cancer.

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Authors and Affiliations

  1. Department of Molecular Oncology, U119 Inserm, Institut Paoli-Calmettes, IFR57, Marseille, France

    Bénédicte Delaval, Alexia Ferrand, Nathalie Conte & Daniel Birnbaum

  2. E229 Inserm, CRLC Val d’Aurelle/Paul Lamarque, Montpellier, France

    Christian Larroque

  3. CNRS, Institut Jacques Monod, Paris, France

    Danièle Hernandez-Verdun

  4. Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS, Université de Rennes 1, IFR 97, Faculté de Médecine, Rennes, France

    Claude Prigent

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  1. Bénédicte Delaval
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  2. Alexia Ferrand
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Correspondence to Daniel Birnbaum.

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Delaval, B., Ferrand, A., Conte, N. et al. Aurora B -TACC1 protein complex in cytokinesis. Oncogene 23, 4516–4522 (2004). https://doi.org/10.1038/sj.onc.1207593

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