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Binding of RNA to p53 regulates its oligomerization and DNA-binding activity

Oncogene volume 23, pages 4371–4379 (2004)Cite this article

Abstract

The C-terminus of p53 is responsible for maintaining the latent, non-DNA-binding form of p53. However, the mechanism by which the C-terminus regulates DNA binding is not yet fully understood. We show here that p53 interacts with RNA via its C-terminal domain and that disruption of this interaction, by RNase A treatment, truncation or phosphorylation of the C-terminus, restores DNA-binding activity. Furthermore, the oligomerization of p53 is significantly enhanced by disrupting the interaction between p53 and RNA. These findings suggest that binding of RNA to p53 is involved in the mechanism of p53 latency.

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Acknowledgements

This work was supported by MEXT. KAKENHI (13218132).

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Authors and Affiliations

  1. Department of Molecular Biology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Fukuoka, Japan

    Yoichiro Yoshida, Hiroto Izumi, Takayuki Torigoe, Hiroshi Ishiguchi, Takeshi Yoshida & Kimitoshi Kohno

  2. Department of Surgery I, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Fukuoka, Japan

    Hideaki Itoh

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  1. Yoichiro Yoshida
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  2. Hiroto Izumi
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Correspondence to Kimitoshi Kohno.

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Yoshida, Y., Izumi, H., Torigoe, T. et al. Binding of RNA to p53 regulates its oligomerization and DNA-binding activity. Oncogene 23, 4371–4379 (2004). https://doi.org/10.1038/sj.onc.1207583

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