Figure 4 | Oncogene

Figure 4

From: Promoter-specific p53-dependent histone acetylation following DNA damage

Figure 4

Mutation of p53 binding sites in the p21 promoter. A lentiviral vector containing a fragment of the human p21 promoter was integrated into mouse F9 cells, which contain wild-type p53. (a) Schematic diagram showing the structure of the lentiviral vector. The construct contains the proximal, distal, and VitD amplicons shown in Figure 3. (b) Residues mutated to abolish p53 binding to the proximal and distal p53 response elements. Residues matching the p53 consensus are in capitals, with the invariant C/G basepairs in bold. (ce) ChIP from transduced F9 cells treated for 9 h with 200 ng/ml doxorubicin. The PCR primers are human-specific and do not amplify endogenous murine DNA. (c) ChIP for p53 binding. (d) ChIP for H4 acetylation. (e) ChIP for H3 acetylation

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