Figure 3 | Oncogene

Figure 3

From: Promoter-specific p53-dependent histone acetylation following DNA damage

Figure 3

p53 binding and histone acetylation at the p21 promoter after 5FU treatment. ChIP from HCT116 wt and p53 knockout cells was used to map p53 binding and histone acetylation at the sites shown in Figure 2. Cells were treated with 375 μ M 5FU for 0, 3, 6, and 9 h before crosslinking. (a) ChIP for p53 binding. (b) ChIP for H4 acetylation. (c) ChIP for H3 acetylation. H4 acetylation is p53-dependent and centred on the p53 binding sites; H3 acetylation is weaker and is seen in the transcribed DNA (intron) even in the absence of p53

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