Figure 2 | Oncogene

Figure 2

From: Promoter-specific p53-dependent histone acetylation following DNA damage

Figure 2

Sites of p53 binding within the p21 promoter. Wt p53 and p53 mutants were expressed from the tet-off promoter in p53-null H1299 cells (Saller et al., 1999). p53 expression was induced by removal of tetracycline from the medium. The negative control cells contain an integrated empty vector (no p53) or express the tumour-derived mutant 175H from the tet-off promoter. p53 binding was measured by ChIP using amplicons located from −3.1 to +0.6 kb relative to the transcription start site. The amplicons labelled upstream, VitD, TATA, and intron do not contain known p53 binding sites. Wt p53 preferentially binds to the distal p53 binding site; the sequence specificity mutant 121F preferentially binds to the proximal site

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