Figure 1 | Oncogene

Figure 1

From: Promoter-specific p53-dependent histone acetylation following DNA damage

Figure 1

p53 binding and histone acetylation at the p21, MDM2, and PUMA promoters. HCT116 colon cancer cells were treated with 375 μ M 5FU to activate p53. (a) Western blot for p53, MDM2, and p21. The loading control is an invariant Ponceau S stained band. (b) ChIP for p53, H3, and H4 acetylation at the indicated times after 5FU treatment. wt, parental HCT116 cells containing wild-type p53; ko, p53 knockout HCT116 cells. The antihistone antibodies recognize multiple acetylated forms of H3 and H4

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