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  • Oncogenomics
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MMP expression profiling in recurred stage IB lung cancer

Oncogene volume 23, pages 845–851 (2004)Cite this article

Abstract

We aimed to clarify the prime role of recurrence in stage I lung cancer. To determine the expression profiles, quantitative RT–PCR and real-time PCR were performed subsequently to evaluate the validity of meaningful molecules identified by 0.12 K c-DNA array experiment surveys. In all, 10 lung cancer patients presenting with recurrence of stage IB were selected and compared with 10 stage IB lung cancer patients without recurrence since biopsied 3 years previously. On c-DNA microarray data analysis using pairs of recurred and the corresponding nonrecurred patients, the following genes were found to be upregulated in the recurred cases: matrix metalloproteinase (MMP)-10 in five cases, MMP-12 in two cases, MMP-11, MMP-14, MMP-15, fos, cyclin E2, E2F3, TGF-α in each one case. The most frequently upregulated genes in recurred lung cancers were MMP-10 (stromelysin-2) and MMP-12 (macrophage elastase). On transcriptional assay by quantitative RT–PCR and real-time RT–PCR analysis to validate those molecules, both transcripts of MMP-10 and MMP-12 were significantly more upregulated in recurred stage IB lung cancer than in the non-recurred stage IB lung cancer (P=0.004). Transcript levels were identical to c-DNA array data. The protein levels of these entities were also evaluated by immunohistochemistry of archival slides. By immunohistochemistry, MMP-10 monoclonal antibody showed more intense immunoreactivity in the recurred stage IB lung cancer than in the nonrecurred stage IB lung cancer (P=0.0313). Our approach revealed that MMP-10 plays an important role in the recurrence in stage IB lung cancer, irrespective of the histologic type.

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References

  • Bolon I, Devouassoux M, Robert C, Moro D, Brambilla C and Brambilla E . (1997). Am. J. Pathol., 150, 1619–1629.

  • Brown PD, Bloxidge RE, Stuart NSA, Gatter KC and Carmichael J . (1993). J. Natl. Cancer Inst., 85, 574–578.

  • Cai M, Onoda K, Takao M, Kyoko I, Shimpo H, Yoshida T and Yada I . (2002). Clin. Cancer Res., 8, 1152–1156.

  • Carmeleit P, Moons L, Lijnen Rn, Crawley J, Tipping P, Drew A, Eeckhout Y, Shapiro SD, Lupu F and Collen D . (1997). Nat Genet, 17, 439–444.

  • Chambers AF and Matrisian LM . (1997). J. Natl. Cancer Inst., 89, 1260–1270.

  • Curran S and Murray GI . (2000). Eur. J. Cancer, 36, 1621–1630.

  • Delebecq TJ, Porte H, Zerimech F, Copin M, Gouyer V, Dacquembronne E, Balduyck M, Wirtz A and Huer G . (2000). Clin. Cancer Res., 6, 1086–1092.

  • Fong KM, Kida Y, Zimmerman PV and Smith PJ . (1996). Clin. Cancer Res., 2, 1369–1372.

  • Hautamaki RD, Kobayashi DK, Senior RM and Shapiro SD . (1997). Science, 277, 2002–2004.

  • Kerkela E, Jeskanen L, Lohi J, Grenman R, Kahari VM and Saarialho-Kere U . (2001). Br. J. Cancer, 84, 650–669.

  • Langmann T, Mauerer R, Zahn A, Moehle C, Probst M, Stermmel W and Schmitz G . (2003). Clin. Chem., 49, 230–238.

  • Martini N, Bains MS, Burt ME, Zakowski MF, Mocormack P, Rusch VW and Ginberg RJ . (1995). J. Thorac. Cardiovasc. Surg., 109, 120–129.

  • Martini N and Beattic Jr EJ . (1977). J. Thorac. Cardiovasc. Surg., 74, 499–505.

  • Muller D, Breathnach R, Millon R, Bronner G, Flesch H, Dumont P, Eber M and Abecassis J . (1991). Int. J. Cancer, 48, 550–556.

  • Murray GI . (2001). J. Pathol., 195, 135–137.

  • Nagakawa H and Yagihashi S . (1994). Jpn. J. Cancer Res., 85, 934–938.

  • Nagase H (ed). (1998). Stromelysin 1 and 2. Matrix Metalloproteinases, Parks WC and Mecham RP (eds). Academic Press: San Diego, pp. 43–84.

    Book  Google Scholar 

  • Nawrocki B, Polette M, Marchand V, Monteau M, Gillery P, Tournier JM and Birembaut P . (1997). Int. J. Cancer, 72, 556–564.

  • Ohori P, Yousem SA, Griffin J, Stanis K, Stetler-Stevenson WG, Colby TV and Somez-Alpan E . (1992). Am. J. Surg. Pathol., 16, 675–686.

  • Passlick B, Sienel W, Seen-Hibler R, Wockel W, Thetter O, Mutschler W and Pantel K . (2000). Clin. Cancer Res., 6, 3944–3948.

  • Rechardt O, Elomaa O, Vaalamo M, Paakkonen K, Jahkola T, Hook-Nikane J, Hembry RM, Hakkinen L, Kere J and Saarialho-Kere U . (2000). J. Invest. Dermatol., 115, 778–787.

  • Shapiro SD and Senior RM . (1998). Macrophage Elastase: Matrix Metalloproteinases, Parks WC Mecham RP (eds). Academic Press: San Diego, pp. 185–197.

    Book  Google Scholar 

  • Soini Y, Paakko P and Autio-Harmainen H . (1993). Am. J. Pathol., 142, 1622–1630.

  • Sreenath T, Matrisian LM, Stetler-Stevenson W, Gattoni-Celli S and Pozzatti RO . (1992). Cancer Res., 52, 4942–4947.

  • Thomas P, Khokha R, Shepherd FA, Feld R and Tsao M . (2000). J. Pathol., 190, 150–156.

  • Tokuraku M, Sato H, Murakami S, Okada Y, Watanabe Y and Seiki M . (1995). Int. J. Cancer, 64, 355–359.

  • Urbanski SJ, Edwards DR, Maitland A, Leco KJ, Watson A and Kossakowska AE . (1992). Br. J. Cancer, 66, 1188–1194.

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Acknowledgements

This study was financially supported by a faculty research grant (CMB-YUHAN 2002-02) of Yonsei University College of Medicine for 2002 [NHC & SHC]. This study was supported by BK 21 project for Medical Science, Yonsei University [NHC].

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Authors and Affiliations

  1. Department of Pathology, Yonsei University College of Medicine, Seoul, Korea

    Nam Hoon Cho, Sung Hui Hong, Suki Kang & Sang Ho Cho

  2. BK 21 project for Medical Science, Yonsei University, Seoul, Korea

    Nam Hoon Cho

  3. Department of Thoracic Surgery, Yonsei University College of Medicine, Seoul, Korea

    Kyi Pyo Hong & Kyung Young Chung

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  1. Nam Hoon Cho
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Correspondence to Sang Ho Cho.

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Cho, N., Hong, K., Hong, S. et al. MMP expression profiling in recurred stage IB lung cancer. Oncogene 23, 845–851 (2004). https://doi.org/10.1038/sj.onc.1207140

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