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Stability and subcellular localization of API2-MALT1 chimeric protein involved in t(11;18) (q21;q21) MALT lymphoma

Oncogene volume 22, pages 8085–8092 (2003)Cite this article

Abstract

t(11; 18) (q21; q21) is a chromosomal aberration specific to low-grade mucosa-associated lymphoid tissue (MALT) lymphoma, and generates the chimeric product apoptosis inhibitor 2 (API2)-MALT1, which has been suggested to play an important role in MALT lymphomagenesis. However, little is known about the characteristics of API2, MALT1, and API2-MALT1 proteins. We therefore investigated the subcellular localization and stability of these products. API2 was localized in the nucleus and the cytoplasm, and MALT1 and API2-MALT1 in the cytoplasm only. Western blot analysis showed that the products of API2 and MALT1 were unstable, while the API2-MALT1 product was stable. The API2 deletion mutants at the end of the C-terminal and the MALT1 deletion mutants at the end of the N-terminal were stable compared with the full-length products. These results indicate that the domains responsible for protein instability are located in the end of the C-terminal of API2 and in that of the N-terminal of MALT1, and also that API2-MALT1 became stable because it lacks these domains. It has been suggested that NF-κB activation plays an important role in the tumorigenesis of MALT lymphoma. Our findings further suggest that the stabilized expression of API2-MALT1 products may continuously stimulate the NF-κB activating pathway, thus leading to MALT lymphomagenesis.

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Authors and Affiliations

  1. Division of Molecular Medicine, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, 464-8681, Nagoya, Japan

    Koh Izumiyama, Masao Nakagawa, Yumiko Kasugai, Ritsuro Suzuki, Hiroko Suzuki, Shinobu Tsuzuki, Yoshitaka Hosokawa & Masao Seto

  2. Department of Gastroenterology and Hematology, Hokkaido University Graduate School of Medicine, Sapporo, 060-8638, Japan

    Masakatsu Yonezumi & Masahiro Asaka

Authors
  1. Koh Izumiyama
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  2. Masao Nakagawa
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  3. Masakatsu Yonezumi
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  4. Yumiko Kasugai
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  5. Ritsuro Suzuki
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  9. Masahiro Asaka
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  10. Masao Seto
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Correspondence to Masao Seto.

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Izumiyama, K., Nakagawa, M., Yonezumi, M. et al. Stability and subcellular localization of API2-MALT1 chimeric protein involved in t(11;18) (q21;q21) MALT lymphoma. Oncogene 22, 8085–8092 (2003). https://doi.org/10.1038/sj.onc.1207002

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