Figure 5 | Oncogene

Figure 5

From: MALAT-1, a novel noncoding RNA, and thymosin β4 predict metastasis and survival in early-stage non-small cell lung cancer

Figure 5

MALAT-1 in non-small cell lung cancer. (a) For in depth analysis of expression of the full-length MALAT-1 RNA, we designed three different primer pairs for MALAT-1 for real-time quantitative RT–PCR. The three amplicons covered the 5′ terminal part, the middle part and the 3′ terminal part of MALAT-1 and were named accordingly. The 5′ terminal primers were located within the region identified by subtractive hybridization and were used in the previous experiments. (b) Expression of MALAT-1 was detectable in NSCLC cell lines, and expression levels of all three amplicons correlated significantly (P=0.01–0.005) with each other in five NSCLC cell lines (A549, HTB-53, HTB-56, HTB-57, HTB-58). (c) Comparative genomic hybridization of 23 patients suffering from early-stage NSCLC (Adeno, SCC) revealed a loss of 11q in one and a gain of 11q in five patients. Gain of 11q was associated with higher expression of MALAT-1, whereas loss of 11q led to decreased MALAT-1 expression. (d) MALAT-1 expression was analysed by real-time quantitative RT–PCR in stage I NSCLC patients (only Adeno and squamous cell carcinoma, n=30). Boxplots show the range of MALAT-1 expression levels in samples from nonmetastasizing (n=22) and metastasizing (n=8) patients. Analyses of all three amplicons led to comparable results with higher MALAT-1 expression in the metastasizing samples (P=0.002). (e) Comparison of the mean expression levels from the analysis described in Figure (d) clearly showed higher MALAT-1 expression in the metastasizing group than in the nonmetastasizing group

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