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A major role for Mcl-1 antiapoptotic protein in the IL-6-induced survival of human myeloma cells

Oncogene volume 22, pages 2950–2959 (2003)Cite this article

Abstract

Interleukin-6 (IL-6) is a major survival factor for malignant plasma cells involved in multiple myeloma. Using an RNase protection assay, we looked for gene expression of 10 anti- and proapoptotic Bcl-2-family proteins in 12 IL-6-dependent human myeloma cell lines (HMCL). A high Mcl-1 gene expression was found in all HMCLs and the other genes were variably expressed. Out of the 10 Bcl-2-family members, only the Mcl-1 gene was regulated by IL-6. Upon starvation of IL-6, Mcl-1 gene expression decreased in association with myeloma cell apoptosis and was upregulated after adding IL-6 again in association with myeloma cell survival. A constitutive Mcl-1 expression was induced with an Mcl-1–GFP retrovirus in two IL-6-dependent HMCLs. The Mcl-1 HMCLs have a marked reduced apoptosis upon IL-6 starvation compared to HMCLs transduced with control GFP retrovirus and may grow without adding IL-6. These data emphasize the major role of Mcl-1 antiapoptotic protein in the IL-6-induced survival of human myeloma cells.

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Acknowledgements

This work was supported by a grant from La Ligue contre le Cancer (Equipe Labellisée) (Paris, France).

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  1. 1NSERM U475 and Unit for Cellular Therapy, CHU Montpellier, Montpellier, 34197, France

    Michel Jourdan, Jean-Luc Veyrune, John De Vos, Nicole Redal, Guilhem Couderc & Bernard Klein

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  1. Michel Jourdan
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Correspondence to Bernard Klein.

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Jourdan, M., Veyrune, JL., Vos, J. et al. A major role for Mcl-1 antiapoptotic protein in the IL-6-induced survival of human myeloma cells. Oncogene 22, 2950–2959 (2003). https://doi.org/10.1038/sj.onc.1206423

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