Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Oncogenomics
  • Published:

Fusion of an AF4-related gene, LAF4, to MLL in childhood acute lymphoblastic leukemia with t(2;11)(q11;q23)

Oncogene volume 22, pages 2851–2855 (2003)Cite this article

Abstract

We showed that the LAF4 gene on 2q11.2–12 was fused to the MLL gene on 11q23 in a pediatric patient with CD10 positive acute lymphoblastic leukemia (ALL) having t(2;11)(q11;q23). The LAF4 gene, which encodes a lymphoid nuclear protein of 1227 amino acids with transactivation potential, is thought to have a role in early lymphoid development. The LAF4 protein was homologous to AF4 and AF5q31 proteins that are fused to MLL in infant early pre-B ALL and the breakpoint of LAF4 was located within the region homologous to the transactivation domain of AF4 and AF5q31. Expression of the 8.5-kb LAF4 transcript was detected in the adult heart, brain, and placenta and in the fetal brain. LAF4 expression was found to be higher in ALL cell lines than in AML and Epstein–Barr virus-transformed B-lymphocyte cell lines. These findings suggest that LAF4, AF4 and AF5q31 might define a new family particularly involved in the pathogenesis of 11q23-associated ALL.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4

Similar content being viewed by others

Abbreviations

AML:

acute myeloid leukemia

ALL:

acute lymphoblastic leukemia

RT–PCR:

reverse transcription–polymerase chain reaction

EBV-B:

EBV-transformed B lymphocyte

FISH:

fluorescence in situ hybridization

References

  • Ferrando AA and Look AT . (2000). Semin. Hematol., 37, 381–395.

  • Gecz J, Gedeon AK, Sutherland GR and Mulley JC . (1996). Nat. Genet., 13, 105–108.

  • Gu Y, Nakamura T, Alder H, Prasad R, Canaani O, Cimino G, Croce CM and Canaani E . (1992). Cell, 71, 701–708.

  • Gu Y, Shen Y, Gibbs RA and Nelson DL . (1996). Nat. Genet., 13, 109–113.

  • Hayashi Y . (2000). Semin. Hematol., 37, 368–380.

  • Hayashi Y, Honma Y, Niitsu N, Taki T, Bessho F, Sako M, Mori T, Yanagisawa M, Tsuji K and Nakahata T . (2000). Cancer Res., 60, 1139–1145.

  • Heerema NA, Sather HN, Ge J, Arthur DC, Hilden JM, Trigg ME and Reaman GH . (1999). Leukemia, 13, 679–686.

  • Hunger SP, Tkachuk DC, Amylon MD, Link MP, Carroll AJ, Welborn JL, Willman CL and Cleary ML . (1993). Blood, 81, 3197–3203.

  • Isnard P, Core N, Naquet P and Djabali M . (2000). Blood, 96, 705–710.

  • Ma C and Staudt LM . (1996). Blood, 87, 734–745.

  • Niitsu N, Hayashi Y, Sugita K and Honma Y . (2001). Br. J. Haematol., 112, 315–326.

  • Ono R, Taki T, Taketani T, Kawaguchi H, Taniwaki M, Okamura T, Kawa K, Hanada R, Kobayashi M and Hayashi Y . (2002). Cancer Res., 62, 333–337.

  • Pui CH . (1995). N. Engl. J. Med., 332, 1618–1630.

  • Pui CH, Gaynon PS, Boyett JM, Chessells JM, Baruchel A, Kamps W, Silverman LB, Biondi A, Harms DO, Vilmer E, Schrappe M and Camitta B . (2002). Lancet, 359, 1909–1915.

  • Reichel M, Gillert E, Angermuller S, Hensel JP, Heidel F, Lode M, Leis T, Biondi A, Haas OA, Strehl S, Panzer-Grumayer ER, Griesinger F, Beck JD, Greil J, Fey GH, Uckun FM and Marschalek R . (2001). Oncogene, 20, 2900–2907.

  • Rowley JD . (1998). Annu. Rev. Genet., 32, 495–519.

  • Rubnitz JE, Link MP, Shuster JJ, Carroll AJ, Hakami N, Frankel LS, Pullen DJ and Cleary ML . (1994). Blood, 84, 570–573.

  • Shibuya N, Taki T, Mugishima H, Chin M, Tsuchida M, Sako M, Kawa K, Ishii E, Miura I, Yanagisawa M and, Hayashi Y . (2001). Genes Chromosomes Cancer, 32, 1–10.

  • Super HJ, McCabe NR, Thirman MJ, Larson RA, Le Beau MM, Pedersen-Bjergaard J, Philip P, Diaz MO and Rowley JD . (1993). Blood, 82, 3705–3711.

  • Taketani T, Taki T, Shibuya N, Ito E, Kitazawa J, Terui K and Hayashi Y . (2002). Cancer Res., 62, 33–37.

  • Taki T, Ida K, Bessho E, Hanada R, Kikuchi A, Yamamoto K, Sako M, Tsuchida M, Seto M, Ueda R and Hayashi Y . (1996). Leukemia, 10, 1303–1307.

  • Taki T, Kano H, Taniwaki M, Sako M, Yanagisawa M and, Hayashi Y . (1999). Proc. Natl. Acad. Sci. USA, 96, 14535–14540.

  • Tkachuk DC, Kohler S and Cleary ML . (1992). Cell, 71, 691–700.

Download references

Acknowledgements

We express our appreciation to Shoko Sohma, Hisae Soga, Yumiko Taketani (University of Tokyo) and Yukiko Konno (Dokkyo University School of Medicine) for technical assistance. We also thank Dr Kanji Sugita (Department of Pediatrics, Yamanashi University School of Medicine, Yamanashi, Japan) for providing ALL (KOCL-33, 44, 45) cell lines, and Dr Yoshinobu Matsuo (Hayashibara Biochemical Laboratories, Inc., Fujisaki Cell Center, Okayama, Japan) for providing varieties of ALL cell lines.

Author information

Author notes

  1. Yasuhide Hayashi: To whom requests for reprints should be addressed: Department of Pediatrics, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan. TEL: 81-3-3815-5411 ext. 33452 or 33456; FAX: 81-3-3816-4108; E-mail: hayashi-tky@umin.ac.jp

Authors and Affiliations

  1. Department of Pediatrics, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Mitsuteru Hiwatari, Tomohiko Taki, Takeshi Taketani, Kohmei Ida & Yasuhide Hayashi

  2. Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, 602-8655, Japan

    Masafumi Taniwaki

  3. Department of Pediatrics, Dokkyo University School of Medicine, 880-Kitakobayashi, Mibu, Tochigi, 321-0293, Japan

    Kenichi Sugita, Mayuko Okuya & Mitsuoki Eguchi

Authors
  1. Mitsuteru Hiwatari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tomohiko Taki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takeshi Taketani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Masafumi Taniwaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kenichi Sugita
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mayuko Okuya
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mitsuoki Eguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Kohmei Ida
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yasuhide Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yasuhide Hayashi.

Additional information

Supported by a Grant-in-Aid for Cancer Research from the Ministry of Health, Labor and Welfare of Japan, a Grant-in-Aid for Scientific Research on Priority Areas, and Uehara Memorial Foundation Grant-in-Aid for Scientific Research (B) and (C) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hiwatari, M., Taki, T., Taketani, T. et al. Fusion of an AF4-related gene, LAF4, to MLL in childhood acute lymphoblastic leukemia with t(2;11)(q11;q23). Oncogene 22, 2851–2855 (2003). https://doi.org/10.1038/sj.onc.1206389

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1206389

Keywords

This article is cited by

Search

Advanced search

Quick links