Figure 8 | Oncogene

Figure 8

From: ΔMEKK3:ER* activation induces a p38α/β2-dependent cell cycle arrest at the G2 checkpoint

Figure 8

RM3.21 cells display ΔMEKK3:ER* mediated CDK inhibition in the absence of p21CIP1. (a) RM3.21 and CM3.3 cells were simultaneously released from S phase in the presence of 100 nM 4-HT for 15 and 25 h. The relative induction of p21CIP1 in the two cell lines was compared by immunoblotting equal quantities of whole cell lysates for p21CIP1; total ERK1/2 served as a loading control. (b) RM3.21 cells were treated with the indicated concentrations of 5-aza-2′-deoxycytidine and whole cell lysates were immunoblotted for expression of p21CIP1 and total ERK1/2. (c) RM3.21 cells were released from S phase in the presence of 100 nM 4-HT or ethanol for the indicated timepoints and cyclin A- and cyclin B1-associated kinase activities determined as described in the Materials and methods section. Results are taken from a single experiment representative of three giving similar results

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