Figure 7 | Oncogene

Figure 7

From: ΔMEKK3:ER* activation induces a p38α/β2-dependent cell cycle arrest at the G2 checkpoint

Figure 7

CM3.3 cells fail to induce p53 or p21CIP1 in response to DNA damage. (a) Asynchronous HCT116 (WT), HCT116 (p53−/−) and CM3.3 cells were treated with vehicle control (C), 1 μM Doxorubicin (D) or 100 nM 4-hydroxytamoxifen (4-HT) for 18 h. Whole cell lysates were fractionated by SDS–PAGE and subjected to immunoblotting with antibodies for p53 and p21CIP1. (b) Cycling HCT116 or CM3.3 cells were treated with vehicle or Dox for 18 h and were then stained with propidium iodide prior to analysis of cell cycle profiles

Back to article page