Figure 6 | Oncogene

Figure 6

From: ΔMEKK3:ER* activation induces a p38α/β2-dependent cell cycle arrest at the G2 checkpoint

Figure 6

Activation of ΔMEKK3:ER* in CM3.3 cells results in down-regulation of cyclins A and B, inhibition of CDK2 and CDK1 and an increase in p21CIP1. Aphidicolin synchronized CM3.3 cells were released for the specified periods of time in the presence of 100 nM 4-HT or ethanol. (a) Whole cell lysates were immunoblotted for cyclin A, CDK2, cyclin B1, CDK1, phospho-CDK1Y15 and total ERK1/2 as a loading control. (b) Normalized cell lysates were used to immunoprecipitate either cyclin A or cyclin B and the associated kinase activities were assayed as described in the Materials and methods. (c) Whole cell lysates were immunoblotted for p53 and p21CIP1 (upper panel) whilst CDK2 and CDK1 immunecomplexes were immunoblotted for the presence of p21CIP1 and the relevant CDK

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