Figure 3 | Oncogene

Figure 3

From: ΔMEKK3:ER* activation induces a p38α/β2-dependent cell cycle arrest at the G2 checkpoint

Figure 3

Activation of ΔMEKK3:ER* inhibits cell proliferation. (a) CCl39 cells were transfected with either pBabePuroΔMEKK3:ER* (top panel) or a catalytically inactive version (KD, bottom panel). Twenty-four hours post-transfection, the cell population was divided, replated and selected in puromycin-containing media±1 μM 4-HT for 2 weeks. Surviving colonies were fixed and stained with crystal violet. (b) Individual, asynchronous CM3 clones (CM3.KD, CM3.3, CM3.6 and CM3.7) were treated with increasing concentrations of 4-HT for 72 h before cellular proliferation was analysed by measuring the absorbance of crystal violet stained protein at 590 nm. Data are expressed as mean±range of duplicate samples. (c) CM3.3, CM3.6 and CM3.7 cells were treated with 4-HT for 2 h and JNK1 and p38α activities were measured by immune complex kinase assay. Data is expressed as fold activation relative to the background signal obtained in ethanol (vehicle control) treated cell extracts. Similar results were observed in three independent experiments

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