Figure 2 | Oncogene

Figure 2

From: ΔMEKK3:ER* activation induces a p38α/β2-dependent cell cycle arrest at the G2 checkpoint

Figure 2

Activation of MAPK and SAPK by ΔMEKK3:ER* and cellular stresses in cycling cells. Asynchronous CM3.3 cells (a) and RM3.21 cells (b) growing in 10% (v/v) FBS, were stimulated with 100 nM 4-HT, 10 μg/ml sodium arsenite (Na As), 1 mM hydrogen peroxide or 30 μM anisomycin for 1 h, 10% FBS for 10 min or exposure to 40 mJ UV followed by a 1 h recovery. In all cases ERK1, JNK1 and p38α activities were assayed by immune complex kinase assay. Data is expressed as fold activation relative to the background signal obtained in ethanol (vehicle control) treated cell extracts. Similar results were observed in three independent experiments

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