Figure 1 | Oncogene

Figure 1

From: ΔMEKK3:ER* activation induces a p38α/β2-dependent cell cycle arrest at the G2 checkpoint

Figure 1

Characterization of ΔMEKK3:ER*-induced signalling in CCl39 cells. (a) CCl39 cells or CM3 clones were treated with 4-HT or ethanol vehicle control for 24 h and whole lysates were immunoblotted with the 9E10 monoclonal antibody to reveal expression of Myc-tagged ΔMEKK3:ER*. (b) Serum-starved CM3.3 cells were stimulated 100 nM 4-HT (closed symbols) or ethanol vehicle control (open symbols) for the indicated times and ERK1, JNK1 or p38α activity was assayed by immune complex kinase assay. (c) Serum-starved CM3.3 and CM3.KD cells were stimulated with 100 nM 4-HT for 2 h and ERK1 (open bars), JNK1 (closed bars) and p38a (hatched bars) were assayed by immune complex kinase assay. (d) Serum-starved CM3.3 cells were treated with increasing concentrations of 4-HT for 2 h and ERK1, JNK1 or p38α activity was assayed by immune complex kinase assay. (e) Serum-starved CM3.3 cells were stimulated with 100 nM 4-HT for the times indicated and whole cell lysates were immunoblotted with the 9E10 monoclonal antibody to detect Myc-tagged ΔMEKK3:ER* and total or phospho-Ser73 c-Jun. In all cases results are shown from a single representative experiment and similar results were obtained in two further independent experiments. In addition, these results were reproducible in other independently derived CM3 clones

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