Figure 4 | Oncogene

Figure 4

From: Tuberin, the tuberous sclerosis complex 2 tumor suppressor gene product, regulates Rho activation, cell adhesion and migration

Figure 4

Tuberin expression increases attachment in MDCK and ELT3 cells. Attachment at 30 min after replating was assayed and normalized to 100% attachment at 6 h after plating (2.5×104 cells/well). Each experiment was performed in triplicate. Error bars indicate standard deviation. (a) MDCK cell lines expressing human tuberin, T1 and T2, have increased attachment compared to both vector cell lines, P2 and P3 (*P<0.05). Cell line T8 has increased attachment (#P<0.05) compared to vector cell line P3, but not to vector cell line P2. The average attachment (OD570, see Materials and methods) of vector control cell lines P2 and P3 was 0.265±0.042, compared to 0.406±0.032 of the tuberin-expressing cell lines T1, T2 and T8 (P<0.05). (b) Attachment of tuberin-expressing ELT3 cells, T3 and T4, is increased compared to both vector cell lines, V1 and V3 (*P<0.05). The average attachment (OD570) of vector control cell lines V1 and V3 was 0.324±0.029, compared to 0.482±0.075 of the tuberin-expressing cell lines T3, T4 and T9 (P<0.05)

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