Figure 2 | Oncogene

Figure 2

From: Tuberin, the tuberous sclerosis complex 2 tumor suppressor gene product, regulates Rho activation, cell adhesion and migration

Figure 2

Expression of tuberin activates Rho. Cells (70% confluency) were serum-starved for 16 h before the assay. GTP-Rho was pulled down from 750 μg protein lysate using GST-Rhotekin beads, and immunoblotted using anti-Rho. Total Rho in the whole cell lysate is also shown. Results shown are representative of three independent experiments. Densitometry was used to measure Rho activation. (a) MDCK cells expressing tuberin (T1, T2 and T8) have a threefold relative increase in GTP-Rho compared to vector control cells P2 and P3 (average Rho activation is 3.8 and 1.25, respectively). (b) ELT3 cells expressing tuberin (T3 and T9) have a 2.9-fold relative increase in GTP-bound Rho compared to pMSCVneo vector control cell lines V1 and V3 (average Rho activation is 3.75 and 1.3, respectively)

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