Figure 1 | Oncogene

Figure 1

From: Tuberin, the tuberous sclerosis complex 2 tumor suppressor gene product, regulates Rho activation, cell adhesion and migration

Figure 1

Development of MDCK and ELT3 cells with stable human tuberin expression. (a) Western immunoblot analysis of cell lysates from MDCK cells. P2 and P3 are control cell lines transduced with pMSCVneo. T1, T2 and T8 are tuberin expressing cell lines. Beta-actin is used as loading control. (b) Western immunoblot analysis of cell lysates from ELT3 cells. U are untransduced ELT3 cells. T3, T4 and T9 are tuberin-expressing stable cell lines. V1 and V3 are control cell lines transduced with pMSCVneo. A non-specific cross-reactive band (*) of slower mobility was observed, which was present in other rat cell lines but absent in human cell lines. Pre-incubation with the peptide against which the tuberin antibody was generated, completely blocked the tuberin band, but not the cross-reactive band (data not shown). Beta-actin is used as loading control. (c) Tuberin expression does not stabilize hamartin in ELT3 cells. Western immunoblot analysis of cell lysates from vector control (V1 and V3) and tuberin-expressing (T3, T4 and T9) ELT3 cell lines. Beta-actin was used as loading control

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