Abstract
PAPIN has six PDZ domains and interacts with p0071, a catenin-related protein. Recent studies have revealed that catenins determine the subcellular localization of some PDZ proteins. We have examined whether the localization of PAPIN is determined by p0071 in epithelial cells. PAPIN was localized not only on the lateral membrane but also on the apical membrane, where p0071 was absent. The targeting to both membranes was mediated by the middle region of PAPIN and did not require the p0071-interacting PDZ domain. In cells that came into contact, PAPIN was diffusely distributed on the plasma membrane, while p0071 was concentrated at immature cell–cell contacts. When epithelial cells were exposed to the low concentration of calcium, p0071 was internalized, whereas PAPIN remained on the plasma membrane. We also confirmed that the interaction with p0071 was not essential for the membrane targeting of ERBIN, a recently identified p0071- and ErbB2-binding protein. PAPIN, p0071, and ERBIN formed a complex in 293T cells. Furthermore, ERBIN and ErbB2 were colocalized with PAPIN on the lateral membrane. These findings suggest that PAPIN, p0071, and ERBIN come to the cell–cell contacts independently and interact with each other on the lateral membrane.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Apperson ML, Moon IS, Kennedy MB . 1996 J. Neurosci. 16: 6839–6852
Bilder D, Perrimon N . 2000 Nature 403: 676–680
Borg JP, Marchetto S, Le Bivic A, Ollendorff V, Jaulin-Bastard F, Saito H, Fournier E, Adelaide J, Margolis B, Birnbaum D . 2000 Nat. Cell. Biol. 2: 407–414
Bryant PJ, Huwe A . 2000 Nat. Cell. Biol. 2: E141–E143
Chaib H, Rubin MA, Mucci NR, Li L, Taylor JMG, Day ML, Rhim JS, Macoska JA . 2001 Cancer Res. 61: 2390–2394
Deguchi M, Iizuka T, Hata Y, Nishimura W, Hirao K, Yao I, Kawabe H, Takai Y . 2000 J. Biol. Chem. 275: 29875–29880
Dobrosotskaya IY, Guy RK, James GL . 1997 J. Biol. Chem. 272: 31589–31597
Dobrosotskaya IY, James GL . 2000 Biochem. Biophys. Res. Commun. 270: 903–909
Dobrosotskaya IY . 2001 Biochem. Biophys. Res. Commun. 18: 969–975
Hatzfeld M, Nachtsheim C . 1996 J. Cell. Sci. 109: 2767–2778
Hirao K, Hata Y, Ide N, Takeuchi M, Irie M, Yao I, Deguchi M, Toyoda A, Sudhof TC, Takai Y . 1998 J. Biol. Chem. 273: 21105–21110
Ide N, Hata Y, Nishioka H, Hirao K, Yao I, Deguchi M, Mizoguchi A, Nishimori H, Tokino T, Nakamura Y, Takai Y . 1999 Oncogene 16: 7810–7815
Izawa I, Nishizawa M, Ohtakara K, Inagaki M . 2002a J. Biol. Chem. 277: 5345–5350
Izawa I, Nishizawa M, Tomono Y, Ohtakara K, Takahashi T, Inagaki M . 2002b Genes Cells. 7: 475–485
Jaulin-Bastard F, Arsanto JP, Le Bivic A, Navarro C, Vely F, Saito H, Marchetto S, Hatzfeld M, Santoni MJ, Birnbaum D, Borg JP . 2002 J. Biol. Chem. 277: 2869–2875
Kim HG, Kassis J, Souto JC, Turner T, Wells A . 1999 Histol. Histopathol. 14: 1175–1182
Mino A, Ohtsuka T, Inoue E, Takai Y . 2000 Genes Cells. 5: 1009–1016
Nagafuchi A . 2001 Curr. Opin. Cell. Biol. 13: 600–603
Nishimura W, Iizuka T, Hirabayashi S, Tanaka N, Hata Y . 2000 J. Cell. Physiol. 185: 358–365
Nishimura W, Yao I, Iida J, Tanaka N, Hata Y . 2002 J. Neurosci. 22: 757–765
Paffenholz R, Franke WW . 1997 Differentiation 61: 293–304
Patrie KM, Drescher AJ, Goyal M, Wiggins RC, Margolis B . 2001 J. Am. Soc. Nephrol. 12: 667–677
Perego C, Vanoni C, Massari S, Longhi R, Pietrini G . 2000 EMBO J. 19: 3978–3989
Perego C, Vanoni C, Villa A, Longhi R, Kaech SM, Frohli E, Hajnal A, Kim SK, Pietrini G . 1999 EMBO J. 18: 2384–2393
Shiratsuchi T, Futamura M, Oda K, Nishimori H, Nakamura Y, Tokino T . 1998 Biochem. Biophys. Res. Commun. 247: 597–604
Stevenson BR, Keon BH . 1998 Annu. Rev. Cell. Dev. Biol. 14: 89–109
Tsukita S, Furuse M, Itoh M . 1996 Cell. Struct. Funct. 21: 381–385
Yarden Y, Sliwkowski MX . 2001 Nat. Rev. Mol. Cell. Biol. 2: 127–137
Zhou J, Liyanage U, Medina M, Ho C, Simmons AD, Lovett M, Kosik MS . 1997 NeuroReport 8: 1489–1494
Acknowledgements
This study was supported by grants-in-aids for Scientific Research and Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science, and Technology, a grant from NOVARTIS Foundation (Japan) for the Promotion of Science (1999), and a grant from Yamanouchi Foundation for Research on Metabolic Disorders (2000). We thank Dr Gary Nolan (Stanford University) for phoenix ampho cells. We also thank Ms C Rokukawa and Ms M Miyahara-Tenkatsu for skilful technical assistance.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ohno, H., Hirabayashi, S., Iizuka, T. et al. Localization of p0071-interacting proteins, plakophilin-related armadillo-repeat protein-interacting protein (PAPIN) and ERBIN, in epithelial cells. Oncogene 21, 7042–7049 (2002). https://doi.org/10.1038/sj.onc.1205852
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1205852
Keywords
This article is cited by
-
Heat shock factor 1 ameliorates proteotoxicity in cooperation with the transcription factor NFAT
The EMBO Journal (2010)
-
Protein p0071, an armadillo plaque protein of adherens junctions, is predominantly expressed in distal renal tubules
Histochemistry and Cell Biology (2010)
-
Regulation of Dendrite and Spine Morphogenesis and Plasticity by Catenins
Molecular Neurobiology (2009)
-
Protein p0071, a major plaque protein of non-desmosomal adhering junctions, is a selective cell-type marker
Cell and Tissue Research (2008)
-
Junctional recruitment of mammalian Scribble relies on E-cadherin engagement
Oncogene (2005)