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Gene expression in epithelial ovarian carcinoma

Abstract

We analysed the mRNA levels corresponding to 12 600 transcripts in primary cultures of ovarian epithelial cells derived from nine normal ovaries and 21 epithelial ovarian carcinoma. The class distinction and hierarchical clustering of expression data revealed a clear distinction in gene expression between normal and carcinoma-derived ovarian epithelial cells. Comparison of expression levels revealed 111 genes with mean expression values of >2.5-fold higher in carcinoma cells. Similarly, 62 genes were expressed at >2.5-fold higher levels in normal ovarian epithelial cells. For a few selected genes, we demonstrate that the pattern of differential expression observed in cultured epithelial cells is present in the normal ovaries and epithelial ovarian carcinoma. Use of cultured epithelial cells represents a novel strategy to study gene expression in a cell-type specific manner.

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Acknowledgements

We thank C Denny, S Nelson and S Cole for helpful comments, and Z Chen (UCLA Microarray Core and Gene Expression Core) for assistance with the chip hybridization. This work was supported by grants from the United States Army Medical Research and Materiel Command (DAMD 17919503), NCI Training Grant (T32CA09297) (to D Matei), and an Alfred P Sloan Foundation/DOE postdoctoral fellowship (to TG Graeber).

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Correspondence to David D Chang.

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Matei, D., Graeber, T., Baldwin, R. et al. Gene expression in epithelial ovarian carcinoma. Oncogene 21, 6289–6298 (2002). https://doi.org/10.1038/sj.onc.1205785

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  • DOI: https://doi.org/10.1038/sj.onc.1205785

Keywords

  • ovarian epithelial cells
  • oligonucleotide arrays
  • RT–PCR
  • periostin

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