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Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer

Oncogene volume 21, pages 5631–5642 (2002)Cite this article

Abstract

Previous molecular genetic studies on HeLa cell (a cervical cancer cell line) derived non-tumorigenic and tumorigenic hybrids have localized a tumor suppressor gene to the long arm of chromosome 11. Analysis of cervical cancer cell lines using chromosome 11 specific probes showed deletion and translocation of 11q13 sequences in five out of eight cell lines. Fluorescence in situ hybridization (FISH), using 11q13 specific probes, has shown interstitial deletion of 11q13 sequences in the HeLa cells. In order to determine whether 11q13 deletions occur in primary cervical tumors, we analysed 36 tumors using 20 different microsatellite and RFLP markers. Semi automated fluorescein based allelotyping was performed to identify loss of heterozygosity (LOH) in tumors. The results showed allelic loss in 17 (47%) tumors. Three different regions of loss, one near MEN1, the second near D11S913, and the third near INT2 locus were observed. The smallest region of deletion overlap at the D11S913 locus was localized to a 300 Kb distance between D11S4908 and D11S5023. Fluorescence in situ hybridization (FISH), using 11q13 specific cosmid and BAC (bacterial artificial chromosome) probes, confirmed allelic deletion in the tumors. PCR analysis further identified homozygous deletion of 11q13 sequences in a primary tumor, in HeLa cells and in two HeLa cell derived tumorigenic hybrid cell lines. The homozygous deletion in the cell lines was mapped to a 5.7 kb sequence of 11q13. We hypothesize therefore that a putative cervical cancer tumor suppressor gene exists within the 300 kb of chromosome 11q13.

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Acknowledgements

We thank Mr Jeremy Pay for his laboratory administration assistance and Prof Edward Livingston for his encouragement. This research was supported by the VAGLAHS West Los Angeles Surgical Education and Research program, and by grants from the UCLA Academic Senate, VA administration and California Cancer Research Program to ES Srivatsan, NIH grants CA39312 to JL Redpath and CA19401 to EJ Stanbridge.

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Authors and Affiliations

  1. Department of Surgery, VAGLAHS West Los Angeles, UCLA School of Medicine, Los Angeles, California, 90073, CA, USA

    Eri S Srivatsan, Rita Chakrabarti, Kayvan Zainabadi, Payam Benyamini, Pok Kwan Yang, Kevin Kang, Daria Motamedi, Mark P Sawicki & Rachel A Jesudasan

  2. Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, 20892, Maryland, MD, USA

    Svetlana D Pack & Zhengping Zhuang

  3. Department of Radiation Oncology, Indiana University School of Medicine, Indianapolis, 46202, Indiana, IN, USA

    Marc S Mendonca

  4. Department of Radiation Oncology, UC Irvine, California College of Medicine, Irvine, 92697, California, CA, USA

    Ulla Bengtsson, Chi Sun & J Leslie Redpath

  5. Department of Chemistry and Biochemistry, University of Oklahoma, Norman, 73019, Oklahoma, OK, USA

    Bruce A Roe

  6. Department of Microbiology and Molecular Genetics, UC Irvine, California College of Medicine, Irvine, 92697, California, CA, USA

    Eric J Stanbridge

  7. Department of Anatomic Pathology, City of Hope Medical Center, Duarte, 91010, California, CA, USA

    Sharon P Wilczynski

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  1. Eri S Srivatsan
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Correspondence to Eri S Srivatsan.

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Srivatsan, E., Chakrabarti, R., Zainabadi, K. et al. Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer. Oncogene 21, 5631–5642 (2002). https://doi.org/10.1038/sj.onc.1205698

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  • DOI: https://doi.org/10.1038/sj.onc.1205698

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