Abstract
The high-mobility group A (HMGA) nonhistone chromosomal proteins HMGA1 and HMGA2 play a role in determining chromatin structure and in regulating the transcription of several genes. High levels of these proteins are characteristic of rapidly dividing cells in embryonic tissue and in tumors. The aim of this study was to determine the role of HMGA1 and HMGA2 throughout mouse spermatogenesis. Northern blot analysis and immunocytochemistry showed HMGA1 and HMGA2 expression during the progression from spermatocyte to spermatid. Interestingly, Western blot analysis with antibodies against the HMGA1 gene product revealed only the HMG1c isoform (27 kDa) in the testis; HMGA1a and HMGA1b were undetectable. These three isoforms are encoded by the HMGA1 gene through alternative splicing. Finally, few spermatids and complete absence of spermatozoa were observed in the testes of HMGA2-null mice, which suggests that the HMGA2 gene plays a critical role in male fertility.
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Acknowledgements
This study was supported by the Associazione Italiana per la Ricerca sul Cancro (AIRC), the progetto Finalizzato Biotecnologie of Consiglio Nazionale delle Richerche, and from MURST (ex 40%, Progetto Nazionale Prof. Raffaele Geremia). We are grateful to Jean Ann Gilder for revising and editing the text.
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Chieffi, P., Battista, S., Barchi, M. et al. HMGA1 and HMGA2 protein expression in mouse spermatogenesis. Oncogene 21, 3644–3650 (2002). https://doi.org/10.1038/sj.onc.1205501
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DOI: https://doi.org/10.1038/sj.onc.1205501
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