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Distinction in gene expression profiles of oligodendrogliomas with and without allelic loss of 1p

Oncogene volume 21, pages 3961–3968 (2002)Cite this article

Abstract

Oligodendrogliomas frequently, but not always show sensitivity to chemotherapy and recent studies demonstrated that allelic loss of chromosome 1p is highly associated with this chemosensitivity. To gain insight into the molecular mechanism of such difference, we examined comprehensive gene expression profiles of 11 oligodendroglial tumors, six with and five without 1pLOH (loss of heterozygosity), and two normal brain tissues using the oligonucleotide microarray (GeneChip). Statistically significant numbers of genes were expressed differentially between the two genetic subsets. Clustering analysis separated the tumor subsets well. The tumors with 1pLOH had similar expression profiles to the normal brain for those differentially expressed genes. Many genes showing higher expression in tumors with 1pLOH were presumed to have functions in nervous tissues. Notably, the majority of the 123 genes showing significant expression reduction in tumors with 1pLOH were either on chromosome 1 (50%) or on 19 (10%), and the average expression reduction ratio was about 50% (0.54±0.13) possibly reflecting the chromosomal deletion. Thus, the biological difference between the genetic subsets of oligodendroglioma was indeed reflected to gene expression profile, which provided baseline information for further studies to elucidate the mechanism of chemosensitivity in gliomas.

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Acknowledgements

We thank Drs Yoichi Nakazato, Takanori Hirose, Nobuaki Funada and Junko Hirato for reviewing the histology. We also thank Xijin Ge and Makoto Kano for their help in statistical analysis and Hiroko Meguro for her technical assistance. This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 11671357 and No. 13470184). This study was carried out as a part of The Technology Development for Analysis of Protein Expression and Interaction in Bioconsortia on R&D of New Industrial Science and Technology Frontiers which was performed by Industrial Science, Technology and Environmental Policy Bureau, Ministry of Economy, Trade and Industry, and entrusted by New Energy Development Organization (NEDO).

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Authors and Affiliations

  1. Genome Science Division, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan

    Akitake Mukasa, Shinichi Matsumoto, Shuichi Tsutsumi & Hiroyuki Aburatani

  2. Department of Neurosurgery, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8655, Tokyo, Japan

    Akitake Mukasa, Keisuke Ueki, Akio Asai & Takaaki Kirino

  3. Department of Neurosurgery, Saitama Medical School, 38-2 Morohongo, Moroyama-machi, Iruma-gun, Saitama, 350-0495, Japan

    Ryo Nishikawa

  4. Department of Neurosurgery, School of Medicine, Teikyo University, 2-11-2 Kaga, Itabashi-ku, 173-8606, Tokyo, Japan

    Takamitsu Fujimaki

  5. CREST (Core Research for Evolutional Science and Technology), Japan Science and Technology Corporation, 4-1-8 Honcho, Kawaguchi, 332-0012, Saitama, Japan

    Akio Asai & Takaaki Kirino

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  1. Akitake Mukasa
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Correspondence to Keisuke Ueki or Hiroyuki Aburatani.

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Mukasa, A., Ueki, K., Matsumoto, S. et al. Distinction in gene expression profiles of oligodendrogliomas with and without allelic loss of 1p. Oncogene 21, 3961–3968 (2002). https://doi.org/10.1038/sj.onc.1205495

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