Figure 5 | Oncogene

Figure 5

From: Selenomethionine induction of DNA repair response in human fibroblasts

Figure 5

PCNA repair complex formation enhanced by SeMet. Immune complexes were collected from SeMet-treated or –untreated cells, using an antibody to DNA polymerase epsilon (pol-ε) a known DNA repair protein required for excision repair. Complexes were probed for PCNA content, which was increased in SeMet-treated cells. We could not however detect XPA protein in the pol-ε complex. XPA/XPG complexes were probed for PCNA in SeMet-treated or untreated cells, and PCNA was detected in the complex. ERCC1, a distinct repair protein, was not detected in either complex (not shown). We did observe induction of ERCC1 protein itself by SeMet treatment (open arrow). Immunoglobulin heavy chains were detected in some immune complexes, positions indicated by asterisks. PCNA is indicated by arrows

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