Abstract
We report the genomic organization of a novel human gene mapped to chromosome 6p21, encoding a putative glycosylphosphatidylinositol (GPI) anchored protein containing a MAM (meprin, A5 antigen, protein tyrosine phosphatase μ) domain, that we have termed as GPIM (GPI and MAM) protein. GPIM gene consists of an 8.9 kb transcript composed of 17 coding exons spanning about 65.5 kb of genomic DNA. The deduced polypeptide consists of 955 amino acids and exhibits structural features found in different types of cell adhesion molecules (CAMs), such as the presence of immunoglobulin domains, the presence of a MAM domain or the capacity to anchor to the cell membrane by a GPI motif. Expression analysis in normal human tissues revealed that this gene is expressed as a 5 kb and 9.5 kb mRNA. Furthermore, the smaller transcript is highly expressed in some human cancer cell lines, as well as in different primary tumors (lung, colon, uterus, stomach and breast). Interestingly, the gene was higher expressed in several tumor tissues analysed as compared to their corresponding normal tissues. Thus, GPIM is a novel gene codifying a protein with structural features characteristics of some CAMs, which might be involved in the tumor progression.
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Acknowledgements
This work was supported by grants from Aventis-Pharma, Sanofi-Synthelabo, and grant no. 08.6/0023/1998 from the Comunidad Autónoma de Madrid, Madrid, Spain.
The nucleotide sequence in this study has been submitted to the Genbank Data Library with accession number AF 478693.
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De Juan, C., Iniesta, P., González-Quevedo, R. et al. Genomic organization of a novel glycosylphosphatidylinositol MAM gene expressed in human tissues and tumors. Oncogene 21, 3089–3094 (2002). https://doi.org/10.1038/sj.onc.1205383
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DOI: https://doi.org/10.1038/sj.onc.1205383
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