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Alternative transcripts of the candidate tumor suppressor gene, WWOX, are expressed at high levels in human breast tumors

Oncogene volume 21, pages 1832–1840 (2002)Cite this article

Abstract

The presence of putative tumor-suppressor genes on chromosome 16q23.2-24.1 has been suggested by LOH analysis in several cancer types. This region overlaps with the fragile site FRA16D and the region of homozygous deletions found in several cancer types. The candidate gene WWOX/FOR has been mapped within this region. The mouse homologue of the WWOX protein has been defined as an apoptogenic protein and an essential partner of p53 in cell death, supporting WWOX as a tumor suppressor gene candidate. We performed an expression study of the WWOX/FOR gene in a series of human breast tumors and breast cancer cell lines, and detected reduced expression of the WWOX/FOR transcript in a series of breast cancer cells. Furthermore, identification of two distinct alternative WWOX transcripts expressed at high levels in human tumors suggests an involvement of the WWOX gene in breast cancer progression.

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Abbreviations

LOH:

loss of heterozygosity

PAC:

P1-derived artificial chromosome

EST:

expressed sequence tag

ADH:

alcohol dehydrogenase

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Acknowledgements

We are indebted to the medical and surgical departments of the Centre René Huguenin for their cooperation, and to J Solheim and H Harstad for excellent technical assistance. This work was supported by the Ligue Nationale de Lutte Contre le Cancer (LNCC); the Association pour la Recherche sur le Cancer (ARC), the Research Council of Norway and the Norwegian Cancer Society (EF and HP). K Driouch was supported by a grant from the Ligue Nationale de Lutte Contre le Cancer.

The sequence data described in this paper have been submitted to the GenBank data library under accession numbers AW874683-AW874700, AZ124367-AZ124379, AZ124382.

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Authors and Affiliations

  1. INSERM E 0017/Oncogénétique, Centre René Huguenin, 35 rue Dailly, F-92211 St-Cloud, France

    Keltouma Driouch, Rossana Monese & Rosette Lidereau

  2. Biotechnology Centre of Oslo, University of Oslo, PO Box 1125, Oslo, N-0349, Norway

    Keltouma Driouch, Hans Prydz, Henning Johansen & Eirik Frengen

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  1. Keltouma Driouch
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Correspondence to Keltouma Driouch or Eirik Frengen.

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Driouch, K., Prydz, H., Monese, R. et al. Alternative transcripts of the candidate tumor suppressor gene, WWOX, are expressed at high levels in human breast tumors. Oncogene 21, 1832–1840 (2002). https://doi.org/10.1038/sj.onc.1205273

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