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Feline STK gene expression in mammary carcinomas

Oncogene volume 21, pages 1785–1790 (2002)Cite this article

Abstract

The human RON and its mouse homologue stk are members of the MET family of tyrosine kinase receptors. We have previously shown that the RON gene is over-expressed in human breast carcinomas. As cat mammary tumours have been proposed as a suitable model for aggressive human breast cancer, we identified the feline stk gene and studied its expression in cat mammary cancer. Feline stk sequences were found highly homologous to the stk and RON gene exons that encode the juxtamembrane and transmembrane domains of the stk and RON receptors. Feline stk-specific transcript was detected by RT–PCR in cat lung and in 7/8 feline mammary carcinomas and a synchronous skin metastasis examined. Western blot and immunohistochemical analyses were carried out with an antibody that recognized both the human RON and mouse stk receptors. This antibody specifically detected a 135 Kd feline protein and stained 10/34 mammary carcinoma archival samples. These data show that the pattern of expression and distribution of the stk protein in feline mammary cancer could be superimposed on that of the RON receptor in human breast cancer and suggest that these feline tumours are a suitable model to test innovative approaches to therapy of aggressive human breast carcinomas.

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Acknowledgements

The secretarial help of Mrs Elaine Wright and Mary Lobianco and the technical assistance of sig. Vincenzo De Sio are gratefully acknowledged. This work has been supported by AIRC and MURST Cofin 1999 funding to MF Di Renzo, M Prat, M Castagnaro and PM Comoglio.

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Author notes

  1. Raffaella De Maria and Piera Maggiora: R De Maria and P Maggiora contributed equally and should both be considered first authors

Authors and Affiliations

  1. Comparative Oncology Center, Institute for Cancer Research and Treatment, University of Torino, Km 3.95, Candiolo (Torino), 142, 10060, SP, Italy

    Raffaella De Maria & Piera Maggiora

  2. Laboratory of Cancer Genetics, Institute for Cancer Research and Treatment, University of Torino, SP 142, Km 3.95, Candiolo (Torino), 10060, Italy

    Piera Maggiora & Maria Flavia Di Renzo

  3. Division of Molecular Oncology, Institute for Cancer Research and Treatment (IRCC), University of Torino, SP 142, Km 3.95, Candiolo (Torino), 10060, Italy

    Paolo M Comoglio

  4. Department of Animal Pathology, School of Veterinary Medicine, University of Torino, Torino, Italy

    Raffaella De Maria & Bartolomeo Biolatti

  5. Department of Medical Sciences, School of Medicine, University of Novara, Italy

    Maria Prat

  6. Department of Public Health Comparative Pathology and Veterinary Hygiene, School of Veterinary Medicine, University of Padova, Italy

    Massimo Castagnaro

Authors
  1. Raffaella De Maria
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  2. Piera Maggiora
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  3. Bartolomeo Biolatti
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  4. Maria Prat
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  5. Paolo M Comoglio
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  6. Massimo Castagnaro
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  7. Maria Flavia Di Renzo
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Correspondence to Maria Flavia Di Renzo.

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De Maria, R., Maggiora, P., Biolatti, B. et al. Feline STK gene expression in mammary carcinomas. Oncogene 21, 1785–1790 (2002). https://doi.org/10.1038/sj.onc.1205221

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