Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Short Report
  • Published:

Inhibition of HOS expression and activities by Wnt pathway

Oncogene volume 21, pages 856–860 (2002)Cite this article

Abstract

βTrCP and HOS are closely related F-box proteins, which play key roles in ubiquitination and degradation of β-catenin and IκB through associating with those phosphorylated substrates and recruiting SCF E3 ubiquitin ligase. Here we report that activation of Wnt/β-catenin signal transduction pathway elevates βTrCP levels but inhibits expression of HOS in 293T cells. Similar disparity is likely to exist in human colorectal tumors. In the NIH3T3 cells, which express HOS, but not βTrCP, Wnt/β-catenin signaling leads to inhibition of HOS promoter activity and NF-κB-driven transcription as well as to stabilization of β-catenin. These results indicate that expression and activities of HOS are negatively regulated by Wnt/β-catenin pathway.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3

Similar content being viewed by others

References

  • Bafico A, Gazit A, Wu-Morgan SS, Yaniv A, Aaronson SA . 1998 Oncogene 16: 2819–2825

  • Bour S, Perrin C, Akari H, Strebel K . 2001 J. Biol. Chem. 276: 15920–15928

  • Cenciarelli C, Chiaur DS, Guardavaccaro D, Parks W, Vidal M, Pagano M . 1999 Curr. Biol. 9: 1177–1179

  • Deshaies RJ . 1999 Annu. Rev. Cell. Dev. Biol. 15: 435–467

  • Finco TS, Westwick JK, Norris JL, Beg AA, Der CJ, Baldwin Jr AS . 1997 J. Biol. Chem. 272: 24113–24116

  • Fuchs SY, Chen A, Xiong Y, Pan ZQ, Ronai Z . 1999 Oncogene 18: 2039–2046

  • Fuchs SY, Fried VA, Ronai Z . 1998 Oncogene 17: 1483–1490

  • Hart M, Concordet JP, Lassot I, Albert I, del los Santos R, Durand H, Perret C, Rubinfeld B, Margottin F, Benarous R, Polakis P . 1999 Curr. Biol. 9: 207–210

  • Joazeiro CA, Weissman AM . 2000 Cell. 102: 549–552

  • Kitagawa M, Hatakeyama S, Shirane M, Matsumoto M, Ishida N, Hattori K, Nakamichi I, Kikuchi A, Nakayama K . 1999 EMBO J. 18: 2401–2410

  • Koike J, Sagara N, Kirikoshi H, Takagi A, Miwa T, Hirai M, Katoh M . 2000 Biochem. Biophys. Res. Commun. 269: 103–109

  • Korinek V, Barker N, Morin PJ, van Wichen D, de Weger R, Kinzler KW, Vogelstein B, Clevers H . 1997 Science 275: 1784–1787

  • Kroll M, Margottin F, Kohl A, Renard P, Durand H, Concordet JP, Bachelerie F, Arenzana-Seisdedos F, Benarous R . 1999 J. Biol. Chem. 274: 7941–7945

  • Lassot I, Segeral E, Berlioz-Torrent C, Durand H, Groussin L, Hai T, Benarous R, Margottin-Goguet F . 2001 Mol. Cell. Biol. 21: 2192–2202

  • Latres E, Chiaur DS, Pagano M . 1999 Oncogene 18: 849–854

  • Maniatis T . 1999 Genes Dev. 13: 505–510

  • Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, Kinzler KW . 1997 Science 275: 1787–1790

  • Munemitsu S, Albert I, Rubinfeld B, Polakis P . 1996 Mol. Cell. Biol. 16: 4088–4094

  • Polakis P . 1999 Curr. Opin. Genet. Dev. 9: 15–21

  • Polakis P, Hart M, Rubinfeld B . 1999 Adv. Exp. Med. Biol. 470: 23–32

  • Reuther JY, Reuther GW, Cortez D, Pendergast AM, Baldwin Jr AS . 1998 Genes Dev. 12: 968–981

  • Saitoh T, Katoh M . 2001 Int. J. Oncol. 18: 959–964

  • Spencer E, Jiang J, Chen ZJ . 1999 Genes Dev. 13: 284–294

  • Spiegelman VS, Slaga TJ, Pagano M, Minamoto T, Ronai Z, Fuchs SY . 2000 Mol. Cell. 5: 877–882

  • Spiegelman VS, Stavropoulos P, Latres E, Pagano M, Ronai Z, Slaga TJ, Fuchs SY . 2001 J. Biol. Chem. 24: 24–

  • Suzuki H, Chiba T, Kobayashi M, Takeuchi M, Suzuki T, Ichiyama A, Ikenoue T, Omata M, Furuichi K, Tanaka K . 1999 Biochem. Biophys. Res. Commun. 256: 127–132

  • Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW . 1999a Curr. Biol. 9: 1180–1182

  • Winston JT, Strack P, Beer-Romero P, Chu CY, Elledge SJ, Harper JW . 1999b Genes Dev. 13: 270–283

  • Yaron A, Gonen H, Alkalay I, Hatzubai A, Jung S, Beyth S, Mercurio F, Manning AM, Ciechanover A, Ben-Neriah Y . 1997 EMBO J. 16: 6486–6494

  • Yaron A, Hatzubai A, Davis M, Lavon I, Amit S, Manning AM, Andersen JS, Mann M, Mercurio F, Ben-Neriah Y . 1998 Nature 396: 590–594

Download references

Acknowledgements

We appreciate the comments of unknown reviewers, which helped us to improve this manuscript. We thank P Stavropoulos for constructing the HOS promoter-driven luciferase reporter. We are grateful to Dr F Luca for critical suggestions. We also thank Drs S Aaronson, B Vogelstein, J Manfredi and P Polakis for reagents. The work was supported in part by NIH grants CA 92900 (to SY Fuchs) and CA 76262 (to TJ Slaga).

Author information

Authors and Affiliations

  1. AMC Cancer Research Center, Lakewood, 80214, Colorado, CO, USA

    Vladimir S Spiegelman & Thomas J Slaga

  2. Department of Animal Biology, University of Pennsylvania, Philadelphia, 19104, Pennsylvania, PA, USA

    Weigang Tang & Serge Y Fuchs

  3. Genetics Division, National Cancer Center Research Institute, Tokyo, 104-0045, Japan

    Masaru Katoh

Authors
  1. Vladimir S Spiegelman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Weigang Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masaru Katoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Thomas J Slaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Serge Y Fuchs
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Serge Y Fuchs.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Spiegelman, V., Tang, W., Katoh, M. et al. Inhibition of HOS expression and activities by Wnt pathway. Oncogene 21, 856–860 (2002). https://doi.org/10.1038/sj.onc.1205132

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1205132

Keywords

This article is cited by

Search

Advanced search

Quick links