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The gene for interleukin-21 receptor is the partner of BCL6 in t(3;16)(q27;p11), which is recurrently observed in diffuse large B-cell lymphoma

Oncogene volume 21, pages 368–376 (2002)Cite this article

Abstract

BCL6 translocation affecting the chromosomal band 3q27 can involve a number of non-immunoglobulin (non-IG) gene loci as partners. We report here that the gene for interleukin-21 receptor (IL-21R) is the partner of BCL6 in t(3;16)(q27;p11) translocation. The two breakpoints on 16p11 of a lymphoma cell line YM and case no. 1012 with diffuse large B-cell lymphoma, both of which carried t(3;16), were localized within the ∼27-kb intron 1 of IL-21R. As a result of t(3;16), the promoter region of IL-21R was substituted for the regulatory sequences of BCL6 in the same transcriptional orientation. Reverse transcriptase-mediated polymerase chain reaction revealed chimeric mRNA consisting of two non-coding exons 1a/1b of IL-21R and coding exons of BCL6 in both lymphoma cells. Fluorescence in situ chromosomal hybridization of YM metaphase cells revealed fusion signals that contained both the BCL6 and IL-21R sequences on the der(3)t(3;16) chromosome. IL-21R was actively transcribed in YM cells, while BCL6 that was under the control of the IL-21R promoter was only moderately expressed at the mRNA and protein level. We constructed expression plasmid of BCL6 that followed the promoter sequences of IL-21R. COS-7 cells transiently transfected with the plasmid expressed high level Bcl-6 protein and displayed nuclear staining with a characteristic punctate pattern by immunofluorescence, indicating that expression of BCL6 can be enhanced by t(3;16). This study added to the list of non-IG partners of BCL6 translocations a new class of gene, i.e. cytokine receptor gene, the expression of which is closely associated with lymphoid cells.

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Acknowledgements

The authors thank Dr I Miura of Akita University School of Medicine for cytogenetic analysis of no. 1012 and Dr T Miki of Tokyo Medical and Dental University for providing the BCL6 cDNA clone. This work was supported by Sankyo Foundation of Life Science and grants-in-aid from the Ministry of Education, Science, Sports and Culture (13470204) of Japan.

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Authors and Affiliations

  1. First Division, Department of Internal Medicine, Faculty of Medicine, Kyoto University, 54 Shogoin-Kawaramachi, Sakyo-ku, 606-8507, Kyoto, Japan

    Chiyoko Ueda, Takashi Akasaka, Masayuki Kurata, Yoshitomo Maesako, Momoko Nishikori, Kazunori Imada, Takashi Uchiyama & Hitoshi Ohno

  2. Department of Oral Pathology, Tohoku University School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan

    Ryo Ichinohasama

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Correspondence to Hitoshi Ohno.

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Ueda, C., Akasaka, T., Kurata, M. et al. The gene for interleukin-21 receptor is the partner of BCL6 in t(3;16)(q27;p11), which is recurrently observed in diffuse large B-cell lymphoma. Oncogene 21, 368–376 (2002). https://doi.org/10.1038/sj.onc.1205099

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