Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Short Report
  • Published:

Induction of endogenous β-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice

Oncogene volume 20, pages 7096–7097 (2001)Cite this article

Abstract

Studies of the response of p53-lacZ transgenic mice have uncovered an unexpected induction of endogenous acid-β-galactosidase activity following whole body irradiation. Strong induction of endogenous enzyme activity is seen in a variety of mouse strains commonly used in the production of transgenes. The induction of endogenous enzyme activity therefore complicates the analysis of p53-lacZ transgenes and may also influence the analysis of radiation responses in other lacZ-reporter mice.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  • Gottlieb E, Haffner R, King A, Asher G, Gruss P, Lonai P, Oren M . 1997 EMBO J. 16: 1381–1390

  • Hatton JD, Lin L . 1992 J. Neurosci. Meth. 45: 147–153

  • Hendrikx PJ, Martens ACM, Visser JWM, Hagenbeek A . 1994 Anal. Biochem. 222: 456–460

  • Komarova EA, Chernov MV, Franks R, Wang K, Armin G, Zelnick CR, Chin DM, Bacus SS, Stark GR, Gudkov AV . 1997 EMBO J. 16: 1391–1400

  • Lorimore SA, Coates PJ, Scobie GE, Milne G, Wright EG . 2001 Inflammatory-type responses after exposure to ionizing radiation in vivo: a mechanism for radiation-induced bystander effects? Oncogene 20: 7085–7095

    Article  CAS  Google Scholar 

  • MacCallum DE, Hupp TR, Midgley CA, Stuart D, Campbell SJ, Harper A, Walsh FS, Wright EG, Balmain A, Lane DP, Hall PA . 1996 Oncogene 13: 2576–2587

  • Nowroozi N, Kim S, Gupta A, Warita H, Zernik J . 1998 J. Craniofac. Genet. Dev. Biol. 18: 51–57

  • Weiss DJ, Liggit D, Clark JG . 1999 Histochem. J. 31: 231–236

Download references

Author information

Authors and Affiliations

  1. Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, Dundee, DD1 9SY, UK

    Philip J Coates, Sally A Lorimore, Brigitte A Rigat & Eric G Wright

  2. Department of Surgery and Molecular Oncology, University of Dundee, Ninewells Hospital and Medical School, Dundee, DD1 9SY, UK

    David P Lane

Authors
  1. Philip J Coates
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sally A Lorimore
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Brigitte A Rigat
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. David P Lane
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eric G Wright
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eric G Wright.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Coates, P., Lorimore, S., Rigat, B. et al. Induction of endogenous β-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice. Oncogene 20, 7096–7097 (2001). https://doi.org/10.1038/sj.onc.1204904

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1204904

Keywords

This article is cited by

Search

Advanced search

Quick links