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Overexpression of the thymosin β-10 gene in human ovarian cancer cells disrupts F-actin stress fiber and leads to apoptosis

Oncogene volume 20, pages 6700–6706 (2001)Cite this article

A Corrigendum to this article was published on 13 August 2002

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Abstract

To understand the molecular changes during ovarian cancer development, we profiled differentially expressed genes in five paired normal and cancerous ovarian tissues. Among the genes that showed differential expression, thymosin β-10 expression was decreased in four of five cancer tissues. The decreased level of expression was confirmed by Northern. To investigate the gene's functional role in ovarian cancers, we constructed an adenovirus vector expressing thymosin β-10 and used it to infect ovarian cancer cell lines PA-I and SKOV3. The infected cells showed disrupted F-actin stress fibers, markedly decreased cell growth, and a high rate of apoptosis. Thus, because loss of thymosin β-10 expression may contribute to the development of a subset of ovarian cancers, restoration of thymosin β-10 expression may be a new strategy for ovarian cancer treatment.

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Acknowledgements

This work was partially supported by G7 and SRC funds of the Korea Science and Engineering Foundation and by Texas Tobacco Settlement Funds appropriated by the Texas Legislature to The University of Texas M. D. Anderson Cancer Center.

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Authors and Affiliations

  1. Molecular Therapy Research Center, College of Medicine, Sung Kyun Kwan University, Samsung Medical Center, 50 Ilwon-Dong, Kangnam-Ku, 135-710, Seoul, Korea

    Seung-Hoon Lee, Jung-Joo Choi, Yong-suk Cho, Sun-Hee Lee & Je-Ho Lee

  2. Department of Obstetrics and Gynecology, College of Medicine, Sung Kyun Kwan University, Samsung Medical Center, 50 Ilwon-Dong, Kangnam-Ku, 135-710, Seoul, Korea

    Je-Ho Lee

  3. Cancer Genomics Core Laboratory, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, Texas, USA

    Wei Zhang, Limei Hu & Jie Xu

  4. Department of Pathology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, Texas, USA

    Wei Zhang, Limei Hu, Jie Xu & Jinsong Liu

  5. Department of Pathology and Laboratory Medicine, The University of Pennsylvania, Philadelphia, PA 19104, Pennsylvania, USA

    Jung-Whan Kim

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  1. Seung-Hoon Lee
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Correspondence to Je-Ho Lee.

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Lee, SH., Zhang, W., Choi, JJ. et al. Overexpression of the thymosin β-10 gene in human ovarian cancer cells disrupts F-actin stress fiber and leads to apoptosis. Oncogene 20, 6700–6706 (2001). https://doi.org/10.1038/sj.onc.1204683

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